RRC ID 43263
著者 Yasuda S, Hasui S, Kobayashi M, Itagaki S, Hirano T, Iseki K.
タイトル The mechanism of carrier-mediated transport of folates in BeWo cells: the involvement of heme carrier protein 1 in placental folate transport.
ジャーナル Biosci Biotechnol Biochem
Abstract The aim of this study was to elucidate the mechanism of folate transport in the placenta. A study of folate was carried out to determine which carriers transport folates in the human choriocarcinoma cell line BeWo, a model cell line for the placenta. We investigated the effects of buffer pH and various compounds on folate uptake. In the first part of the study, the expression levels of the mRNA of the folate receptor alpha (FRalpha), the reduced folate carrier (RFC), and heme carrier protein 1 (HCP1) were determined in BeWo cells by RT-PCR analysis. Folate uptake into BeWo cells was greater under an acidic buffer condition than under a neutral one. Structure analogs of folates inhibited folate uptake under all buffer pH conditions, but anion drugs (e.g., pravastatin) inhibited folate uptake only under an acidic buffer condition. Although thiamine pyrophosphate (TPP), a substrate of RFC, had no effect on folate uptake, hemin (a weak inhibitor of folate uptake via HCP1) decreased folate uptake to about 80% of the control level under an acidic buffer condition. Furthermore, kinetic analysis showed that hemin inhibited the low-affinity phase of folate uptake under an acidic buffer condition. We conclude that pH-dependent folate uptake in BeWo cells is mediated by at least two carriers. RFC is not involved in folate uptake, but FRalpha (high affinity phase) and HCP1 (low affinity phase) transport folate in BeWo cells.
巻・号 72(2)
ページ 329-34
公開日 2008-2-1
DOI 10.1271/bbb.70347
PII JST.JSTAGE/bbb/70347
PMID 18256483
MeSH Base Sequence Biological Transport Carrier Proteins / genetics Cell Line, Tumor DNA Primers Female Folate Receptors, GPI-Anchored Folic Acid / metabolism* Humans Hydrogen-Ion Concentration Kinetics Membrane Transport Proteins / physiology* Placenta / metabolism* Pravastatin / pharmacology Proton-Coupled Folate Transporter RNA, Messenger / genetics Receptors, Cell Surface / genetics Reverse Transcriptase Polymerase Chain Reaction Thiamine Pyrophosphate / pharmacology
IF 1.516
引用数 11
WOS 分野 FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 BeWo(RCB1644)