| RRC ID |
43266
|
| Author |
Sato T, Takashiba M, Hayashi R, Zhu X, Yamagata T.
|
| Title |
Glycosylation of dodecyl 2-acetamido-2-deoxy-beta-D-glucopyranoside and dodecyl beta-D-galactopyranosyl-(1-->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside as saccharide primers in cells.
|
| Journal |
Carbohydr Res
|
| Abstract |
Syntheses of oligosaccharides expressed on cells are indispensable for the improvement of the functional analyses of the oligosaccharides and their applications. We are developing saccharide primers for synthesizing oligosaccharides using living cells. In this study, dodecyl 2-acetamido-2-deoxy-beta-D-glucopyranoside (GlcNAc-C12) and dodecyl beta-D-galactopyranosyl-(1-->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (LacNAc-C12) were examined for their abilities to prime the syntheses of neolacto-series oligosaccharides in HL60 cells. When GlcNAc-C12 was incubated with HL60 cells in serum-free medium for 2 days, 14 kinds of glycosylated products were collected from the culture medium. They were separated by high-performance liquid chromatography. The sequences of the products were determined to be neolacto-series oligosaccharides including Lewis(X), sialyl Lewis(X), polylactosamine, and sialylpolylactosamine by mass spectrometry. GlcNAc-C12 was also glycosylated by B16 cells and gave sialyllactosamine. Furthermore, LacNAc-C12 gave similar glycosylated products to GlcNAc-C12.
|
| Volume |
343(5)
|
| Pages |
831-8
|
| Published |
2008-4-7
|
| DOI |
10.1016/j.carres.2008.01.022
|
| PII |
S0008-6215(08)00040-2
|
| PMID |
18262174
|
| MeSH |
Acetylglucosamine / analogs & derivatives
Acetylglucosamine / metabolism*
Acetylglucosamine / pharmacology
Amino Sugars / chemistry
Carbohydrate Sequence
Cell Proliferation / drug effects
Dodecanol / chemistry
Glycosylation
HL-60 Cells
Humans
Lactose / analogs & derivatives
Lactose / metabolism*
Lactose / pharmacology
Molecular Sequence Data
Oligosaccharides / biosynthesis*
Oligosaccharides / chemistry
Oligosaccharides / pharmacology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
|
| IF |
1.841
|
| Times Cited |
12
|
|
WOS Category
|
CHEMISTRY, APPLIED
CHEMISTRY, ORGANIC
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
|
