論文 - 詳細
| RRC ID | 43272 |
|---|---|
| 著者 | Naka T, Iwahashi M, Nakamura M, Ojima T, Nakamori M, Ueda K, Katsuda M, Miyazawa M, Ishida K, Yamaue H. |
| タイトル | Tumor vaccine therapy against recrudescent tumor using dendritic cells simultaneously transfected with tumor RNA and granulocyte macrophage colony-stimulating factor RNA. |
| ジャーナル | Cancer Sci |
| Abstract |
Recently, dendritic cells (DC) transfected with tumor RNA have been used as a cancer vaccine. The efficacy of a cancer vaccine using DC transfected tumor RNA was examined. Of particular interest was whether a vaccine using DC transfected with recrudescent tumor RNA is effective for the treatment of a regrowing tumor after prior immunotherapy. In addition, the usefulness of co-transfection of granulocyte macrophage colony-stimulating factor (GM-CSF) mRNA to augment the DC vaccine was examined. CT26 tumor-bearing mice were immunized by s.c. injection with DC transfected with CT26 mRNA (DC-CT26). The cytotoxic activity against CT26 in mice immunized with DC-CT26 was significantly higher than that in the control group (P < 0.001) and was augmented by GM-CSF mRNA co-transfection (P < 0.05), resulting in remarkable therapeutic efficacy in CT26 s.c. tumor models. Cytotoxic T lymphocytes induced by the vaccination using DC transfected with mRNA from the recrudescent tumor showed a potent cytotoxicity against the recrudescent CT26 tumor cells, which was significantly higher than the cytotoxicity induced by the vaccination using DC-CT26 (P < 0.05). In addition, in a recrudescent tumor model, this vaccination suppressed the regrowing s.c. tumors, and was augmented by GM-CSF mRNA co-transfection (P < 0.05). These results suggested that vaccination therapy using DC simultaneously transfected with whole tumor RNA and GM-CSF mRNA could generate therapeutic immune responses even against recrudescent tumor after prior vaccination. |
| 巻・号 | 99(2) |
| ページ | 407-13 |
| 公開日 | 2008-2-1 |
| DOI | 10.1111/j.1349-7006.2007.00698.x |
| PII | CAS698 |
| PMID | 18271939 |
| MeSH | Animals B7-2 Antigen / immunology Cancer Vaccines / immunology Cancer Vaccines / therapeutic use* Cell Line, Tumor Dendritic Cells / immunology Dendritic Cells / transplantation* Female Granulocyte-Macrophage Colony-Stimulating Factor / genetics* Granulocyte-Macrophage Colony-Stimulating Factor / metabolism Immunotherapy, Adoptive Mice Mice, Inbred BALB C RNA / metabolism RNA / therapeutic use* RNA, Neoplasm / metabolism RNA, Neoplasm / therapeutic use* Receptors, CCR7 / immunology Spleen / metabolism Transfection |
| IF | 4.966 |
| 引用数 | 8 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | Meth-A(RCB0464) |