| RRC ID |
43272
|
| 著者 |
Naka T, Iwahashi M, Nakamura M, Ojima T, Nakamori M, Ueda K, Katsuda M, Miyazawa M, Ishida K, Yamaue H.
|
| タイトル |
Tumor vaccine therapy against recrudescent tumor using dendritic cells simultaneously transfected with tumor RNA and granulocyte macrophage colony-stimulating factor RNA.
|
| ジャーナル |
Cancer Sci
|
| Abstract |
Recently, dendritic cells (DC) transfected with tumor RNA have been used as a cancer vaccine. The efficacy of a cancer vaccine using DC transfected tumor RNA was examined. Of particular interest was whether a vaccine using DC transfected with recrudescent tumor RNA is effective for the treatment of a regrowing tumor after prior immunotherapy. In addition, the usefulness of co-transfection of granulocyte macrophage colony-stimulating factor (GM-CSF) mRNA to augment the DC vaccine was examined. CT26 tumor-bearing mice were immunized by s.c. injection with DC transfected with CT26 mRNA (DC-CT26). The cytotoxic activity against CT26 in mice immunized with DC-CT26 was significantly higher than that in the control group (P < 0.001) and was augmented by GM-CSF mRNA co-transfection (P < 0.05), resulting in remarkable therapeutic efficacy in CT26 s.c. tumor models. Cytotoxic T lymphocytes induced by the vaccination using DC transfected with mRNA from the recrudescent tumor showed a potent cytotoxicity against the recrudescent CT26 tumor cells, which was significantly higher than the cytotoxicity induced by the vaccination using DC-CT26 (P < 0.05). In addition, in a recrudescent tumor model, this vaccination suppressed the regrowing s.c. tumors, and was augmented by GM-CSF mRNA co-transfection (P < 0.05). These results suggested that vaccination therapy using DC simultaneously transfected with whole tumor RNA and GM-CSF mRNA could generate therapeutic immune responses even against recrudescent tumor after prior vaccination.
|
| 巻・号 |
99(2)
|
| ページ |
407-13
|
| 公開日 |
2008-2-1
|
| DOI |
10.1111/j.1349-7006.2007.00698.x
|
| PII |
CAS698
|
| PMID |
18271939
|
| MeSH |
Animals
B7-2 Antigen / immunology
Cancer Vaccines / immunology
Cancer Vaccines / therapeutic use*
Cell Line, Tumor
Dendritic Cells / immunology
Dendritic Cells / transplantation*
Female
Granulocyte-Macrophage Colony-Stimulating Factor / genetics*
Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
Immunotherapy, Adoptive
Mice
Mice, Inbred BALB C
RNA / metabolism
RNA / therapeutic use*
RNA, Neoplasm / metabolism
RNA, Neoplasm / therapeutic use*
Receptors, CCR7 / immunology
Spleen / metabolism
Transfection
|
| IF |
4.966
|
| 引用数 |
8
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Meth-A(RCB0464) |
