Reference - Detail
| RRC ID | 43273 |
|---|---|
| Author | Zhong M, Kadota Y, Shimizu Y, Gohda E. |
| Title | Induction of cytolytic activity and interferon-gamma production in murine natural killer cells by polymyxins B and E. |
| Journal | Int Immunopharmacol |
| Abstract |
Natural killer (NK) cells are the primary effector cells of the innate immune system and have well-established roles in tumor rejection and resistance to viruses, bacteria and certain parasites. There is a need for more specific immune modulators of NK cell activity that lack the wide-ranging side effects of NK cell-stimulatory interleukins. The polycationic antibiotic polymyxin B (PMB) has been shown to have a unique ability to enhance activities of some immune cells, independent of its antibiotic properties. Here we report that both PMB and its analog polymyxin E (PME) markedly enhanced the activity of NK cells enriched from the murine spleen. Maximal activation of NK cell activity was obtained after 24 h of incubation with PMB at a dose of 300 mug/ml. PMB nonapeptide, one of the two PMB domains, and PME methanesulfonate, the negatively charged derivative of PME, had little effect on NK cell activity. PMB induced interferon (IFN)-gamma and tumor necrosis factor-alpha production in NK cells. Proliferation of NK cells in vitro was significantly stimulated by being incubated with PMB. Administration of PMB to mice for 7 consecutive days stimulated splenic NK cell activity and increased NK cell populations in the spleen. These results suggest that the polycationic antibiotics PMB and PME may up-regulate innate and adaptive immune responses by induction of NK cell activity and IFN-gamma production. |
| Volume | 8(3) |
| Pages | 508-13 |
| Published | 2008-3-1 |
| DOI | 10.1016/j.intimp.2007.11.001 |
| PII | S1567-5769(07)00356-6 |
| PMID | 18279806 |
| MeSH | Animals Anti-Bacterial Agents / pharmacology* Colistin / pharmacology* Cytotoxicity, Immunologic / drug effects* Female Interferon-gamma / biosynthesis* Killer Cells, Natural / drug effects* Killer Cells, Natural / immunology Mice Mice, Inbred C57BL Polymyxin B / pharmacology* |
| IF | 3.943 |
| Times Cited | 9 |
| WOS Category | PHARMACOLOGY & PHARMACY IMMUNOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | |