| RRC ID |
43273
|
| Author |
Zhong M, Kadota Y, Shimizu Y, Gohda E.
|
| Title |
Induction of cytolytic activity and interferon-gamma production in murine natural killer cells by polymyxins B and E.
|
| Journal |
Int Immunopharmacol
|
| Abstract |
Natural killer (NK) cells are the primary effector cells of the innate immune system and have well-established roles in tumor rejection and resistance to viruses, bacteria and certain parasites. There is a need for more specific immune modulators of NK cell activity that lack the wide-ranging side effects of NK cell-stimulatory interleukins. The polycationic antibiotic polymyxin B (PMB) has been shown to have a unique ability to enhance activities of some immune cells, independent of its antibiotic properties. Here we report that both PMB and its analog polymyxin E (PME) markedly enhanced the activity of NK cells enriched from the murine spleen. Maximal activation of NK cell activity was obtained after 24 h of incubation with PMB at a dose of 300 mug/ml. PMB nonapeptide, one of the two PMB domains, and PME methanesulfonate, the negatively charged derivative of PME, had little effect on NK cell activity. PMB induced interferon (IFN)-gamma and tumor necrosis factor-alpha production in NK cells. Proliferation of NK cells in vitro was significantly stimulated by being incubated with PMB. Administration of PMB to mice for 7 consecutive days stimulated splenic NK cell activity and increased NK cell populations in the spleen. These results suggest that the polycationic antibiotics PMB and PME may up-regulate innate and adaptive immune responses by induction of NK cell activity and IFN-gamma production.
|
| Volume |
8(3)
|
| Pages |
508-13
|
| Published |
2008-3-1
|
| DOI |
10.1016/j.intimp.2007.11.001
|
| PII |
S1567-5769(07)00356-6
|
| PMID |
18279806
|
| MeSH |
Animals
Anti-Bacterial Agents / pharmacology*
Colistin / pharmacology*
Cytotoxicity, Immunologic / drug effects*
Female
Interferon-gamma / biosynthesis*
Killer Cells, Natural / drug effects*
Killer Cells, Natural / immunology
Mice
Mice, Inbred C57BL
Polymyxin B / pharmacology*
|
| IF |
3.943
|
| Times Cited |
9
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
IMMUNOLOGY
|
| Resource |
| Human and Animal Cells |
|
