RRC ID 43282
Author Zhao J, Ohba S, Shinkai M, Chung UI, Nagamune T.
Title Icariin induces osteogenic differentiation in vitro in a BMP- and Runx2-dependent manner.
Journal Biochem Biophys Res Commun
Abstract To effectively treat bone diseases using bone regenerative medicine, there is an urgent need to develop safe cheap drugs that can potently induce bone formation. Here, we demonstrate the osteogenic effect of icariin, the main active compound of Epimedium pubescens. Icariin induced osteogenic differentiation in pre-osteoblastic MC3T3-E1 cells and mouse primary osteoblasts. Icariin upregulated the mRNA expression levels of the osteoblast marker genes runt-related transcription factor 2 (Runx2) and inhibitor of DNA-binding 1 (Id-1). The osteogenic effect was inhibited by the introduction of Smad6 or dominant-negative Runx2, as well as Noggin treatment. Furthermore, icariin induced the mRNA expression of bone morphogenetic protein (BMP)-4. These data suggest that icariin exerts its potent osteogenic effect through induction of Runx2 expression, production of BMP-4 and activation of BMP signaling. The extremely low cost of icariin and its high abundance make it appealing for bone regenerative medicine.
Volume 369(2)
Pages 444-8
Published 2008-5-2
DOI 10.1016/j.bbrc.2008.02.054
PII S0006-291X(08)00295-7
PMID 18295595
MeSH 3T3-L1 Cells Animals Bone Morphogenetic Protein 4 Bone Morphogenetic Proteins / metabolism* Cell Differentiation / drug effects Core Binding Factor Alpha 1 Subunit / metabolism* Dose-Response Relationship, Drug Drugs, Chinese Herbal / administration & dosage Flavonoids / administration & dosage* Mice Osteoblasts / cytology* Osteoblasts / drug effects Osteoblasts / metabolism* Osteogenesis / drug effects Osteogenesis / physiology*
IF 2.705
Times Cited 120
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells