RRC ID |
43282
|
著者 |
Zhao J, Ohba S, Shinkai M, Chung UI, Nagamune T.
|
タイトル |
Icariin induces osteogenic differentiation in vitro in a BMP- and Runx2-dependent manner.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
To effectively treat bone diseases using bone regenerative medicine, there is an urgent need to develop safe cheap drugs that can potently induce bone formation. Here, we demonstrate the osteogenic effect of icariin, the main active compound of Epimedium pubescens. Icariin induced osteogenic differentiation in pre-osteoblastic MC3T3-E1 cells and mouse primary osteoblasts. Icariin upregulated the mRNA expression levels of the osteoblast marker genes runt-related transcription factor 2 (Runx2) and inhibitor of DNA-binding 1 (Id-1). The osteogenic effect was inhibited by the introduction of Smad6 or dominant-negative Runx2, as well as Noggin treatment. Furthermore, icariin induced the mRNA expression of bone morphogenetic protein (BMP)-4. These data suggest that icariin exerts its potent osteogenic effect through induction of Runx2 expression, production of BMP-4 and activation of BMP signaling. The extremely low cost of icariin and its high abundance make it appealing for bone regenerative medicine.
|
巻・号 |
369(2)
|
ページ |
444-8
|
公開日 |
2008-5-2
|
DOI |
10.1016/j.bbrc.2008.02.054
|
PII |
S0006-291X(08)00295-7
|
PMID |
18295595
|
MeSH |
3T3-L1 Cells
Animals
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins / metabolism*
Cell Differentiation / drug effects
Core Binding Factor Alpha 1 Subunit / metabolism*
Dose-Response Relationship, Drug
Drugs, Chinese Herbal / administration & dosage
Flavonoids / administration & dosage*
Mice
Osteoblasts / cytology*
Osteoblasts / drug effects
Osteoblasts / metabolism*
Osteogenesis / drug effects
Osteogenesis / physiology*
|
IF |
2.985
|
引用数 |
120
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
MC3T3-E1(RCB1126)
10T1/2(RCB0247) |