論文 - 詳細
| RRC ID | 43283 |
|---|---|
| 著者 | Yamahara K, Sone M, Itoh H, Yamashita JK, Yurugi-Kobayashi T, Homma K, Chao TH, Miyashita K, Park K, Oyamada N, Sawada N, Taura D, Fukunaga Y, Tamura N, Nakao K. |
| タイトル | Augmentation of neovascularization [corrected] in hindlimb ischemia by combined transplantation of human embryonic stem cells-derived endothelial and mural cells. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND:We demonstrated that mouse embryonic stem (ES) cells-derived vascular endothelial growth factor receptor-2 (VEGF-R2) positive cells could differentiate into both endothelial cells (EC) and mural cells (MC), and termed them as vascular progenitor cells (VPC). Recently, we have established a method to expand monkey and human ES cells-derived VPC with the proper differentiation stage in a large quantity. Here we investigated the therapeutic potential of human VPC-derived EC and MC for vascular regeneration. METHODS AND RESULTS:After the expansion of human VPC-derived vascular cells, we transplanted these cells to nude mice with hindlimb ischemia. The blood flow recovery and capillary density in ischemic hindlimbs were significantly improved in human VPC-derived EC-transplanted mice, compared to human peripheral and umbilical cord blood-derived endothelial progenitor cells (pEPC and uEPC) transplanted mice. The combined transplantation of human VPC-derived EC and MC synergistically improved blood flow of ischemic hindlimbs remarkably, compared to the single cell transplantations. Transplanted VPC-derived vascular cells were effectively incorporated into host circulating vessels as EC and MC to maintain long-term vascular integrity. CONCLUSIONS:Our findings suggest that the combined transplantation of human ES cells-derived EC and MC can be used as a new promising strategy for therapeutic vascular regeneration in patients with tissue ischemia. |
| 巻・号 | 3(2) |
| ページ | e1666 |
| 公開日 | 2008-2-27 |
| DOI | 10.1371/journal.pone.0001666 |
| PMID | 18301744 |
| PMC | PMC2244708 |
| MeSH | Animals Cell Differentiation Embryonic Stem Cells / cytology Endothelial Cells / transplantation Hindlimb Humans Ischemia / therapy* Mice Mice, Nude Muscle, Smooth, Vascular / cytology Myocytes, Smooth Muscle / cytology Neovascularization, Physiologic* Regional Blood Flow Stem Cell Transplantation / methods* Transplantation, Heterologous |
| IF | 2.74 |
| 引用数 | 75 |
| WOS 分野 | HEMATOLOGY |
| リソース情報 | |
| 研究用ヒト臍帯血幹細胞 | |