Reference - Detail
| RRC ID | 43284 |
|---|---|
| Author | Watanabe S, Wakasugi K. |
| Title | Neuroprotective function of human neuroglobin is correlated with its guanine nucleotide dissociation inhibitor activity. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Mammalian neuroglobin (Ngb) is involved in neuroprotection under oxidative stress conditions such as ischemia and reperfusion. However, the neuroprotective mechanism remains unclear. We previously demonstrated that human ferric Ngb binds to the alpha-subunits of heterotrimeric G proteins (Galpha(i/o)) and acts as a guanine nucleotide dissociation inhibitor (GDI) for Galpha(i/o). In the present study, we used a protein delivery reagent, Chariot, to investigate whether the GDI activity of human Ngb plays an important role in its neuroprotective activity under oxidative stress conditions. We showed that human Ngb mutants, which retained GDI activities, rescued pheochromocytoma PC12 cell death caused by hypoxia/reoxygenation as did human wild-type Ngb. In contrast, zebrafish Ngb and human Ngb mutants, which did not function as GDI proteins, did not rescue cell death. These results clearly show that the GDI activity of human Ngb is tightly correlated with its neuroprotective activity. |
| Volume | 369(2) |
| Pages | 695-700 |
| Published | 2008-5-2 |
| DOI | 10.1016/j.bbrc.2008.02.089 |
| PII | S0006-291X(08)00353-7 |
| PMID | 18302932 |
| MeSH | Animals Apoptosis / physiology* Cell Hypoxia / physiology Globins / metabolism* Guanine Nucleotide Dissociation Inhibitors / metabolism* Humans Nerve Tissue Proteins / metabolism* Neuroglobin Neurons / cytology* Neurons / metabolism* Neuroprotective Agents / metabolism* Oxidative Stress / physiology* PC12 Cells Rats Statistics as Topic |
| IF | 2.985 |
| Times Cited | 45 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | PC-12(RCB0009) |