論文 - 詳細
| RRC ID | 43346 |
|---|---|
| 著者 | Sogo Y, Ito A, Onoguchi M, Oyane A, Tsurushima H, Ichinose N. |
| タイトル | Formation of a FGF-2 and calcium phosphate composite layer on a hydroxyapatite ceramic for promoting bone formation. |
| ジャーナル | Biomed Mater |
| Abstract |
Fibroblast growth factor-2 (FGF-2) was immobilized on a hydroxyapatite (HAP) ceramic in supersaturated calcium phosphate solution prepared using solutions corresponding to clinically approved infusion fluids. To avoid the risk of FGF-2 denaturation, FGF-2 immobilization was carried out at 25 degrees C. FGF-2 was successfully immobilized on HAP ceramic surfaces by deposition with calcium phosphate to form a FGF-2 and calcium phosphate composite layer. A maximum of 2.72 +/- 0.01 microg cm(-2) of FGF-2 was immobilized in the composite layer formed on the HAP ceramic under the optimum condition. A FGF-2-immobilized HAP ceramic is likely to have the ability to release a sufficient amount of FGF-2 to promote bone formation. FGF-2 released from a FGF-2-immobilized HAP ceramic maintained its biological activity, since the proliferation of fibroblastic NIH3T3 was promoted. Therefore, the FGF-2-immobilized HAP ceramic is expected to be a useful material for promoting new bone formation. |
| 巻・号 | 2(3) |
| ページ | S175-80 |
| 公開日 | 2007-9-1 |
| DOI | 10.1088/1748-6041/2/3/S16 |
| PII | S1748-6041(07)49678-4 |
| PMID | 18458464 |
| MeSH | Animals Bone Development / drug effects* Bone Substitutes / administration & dosage* Bone Substitutes / chemistry* Calcium Phosphates / chemistry* Cell Proliferation / drug effects Ceramics / chemistry Durapatite / chemistry* Fibroblast Growth Factor 2 / administration & dosage* Fibroblast Growth Factor 2 / chemistry* Materials Testing Mice NIH 3T3 Cells |
| IF | 3.174 |
| 引用数 | 28 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH3T3-3-4(RCB1862) |