| RRC ID |
43346
|
| 著者 |
Sogo Y, Ito A, Onoguchi M, Oyane A, Tsurushima H, Ichinose N.
|
| タイトル |
Formation of a FGF-2 and calcium phosphate composite layer on a hydroxyapatite ceramic for promoting bone formation.
|
| ジャーナル |
Biomed Mater
|
| Abstract |
Fibroblast growth factor-2 (FGF-2) was immobilized on a hydroxyapatite (HAP) ceramic in supersaturated calcium phosphate solution prepared using solutions corresponding to clinically approved infusion fluids. To avoid the risk of FGF-2 denaturation, FGF-2 immobilization was carried out at 25 degrees C. FGF-2 was successfully immobilized on HAP ceramic surfaces by deposition with calcium phosphate to form a FGF-2 and calcium phosphate composite layer. A maximum of 2.72 +/- 0.01 microg cm(-2) of FGF-2 was immobilized in the composite layer formed on the HAP ceramic under the optimum condition. A FGF-2-immobilized HAP ceramic is likely to have the ability to release a sufficient amount of FGF-2 to promote bone formation. FGF-2 released from a FGF-2-immobilized HAP ceramic maintained its biological activity, since the proliferation of fibroblastic NIH3T3 was promoted. Therefore, the FGF-2-immobilized HAP ceramic is expected to be a useful material for promoting new bone formation.
|
| 巻・号 |
2(3)
|
| ページ |
S175-80
|
| 公開日 |
2007-9-1
|
| DOI |
10.1088/1748-6041/2/3/S16
|
| PII |
S1748-6041(07)49678-4
|
| PMID |
18458464
|
| MeSH |
Animals
Bone Development / drug effects*
Bone Substitutes / administration & dosage*
Bone Substitutes / chemistry*
Calcium Phosphates / chemistry*
Cell Proliferation / drug effects
Ceramics / chemistry
Durapatite / chemistry*
Fibroblast Growth Factor 2 / administration & dosage*
Fibroblast Growth Factor 2 / chemistry*
Materials Testing
Mice
NIH 3T3 Cells
|
| IF |
3.174
|
| 引用数 |
28
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
NIH3T3-3-4(RCB1862) |
