RRC ID 43361
著者 Nagashio R, Sato Y, Jiang SX, Ryuge S, Kodera Y, Maeda T, Nakajima T.
タイトル Detection of tumor-specific autoantibodies in sera of patients with lung cancer.
ジャーナル Lung Cancer
Abstract The presence of autoantibodies (AAs) in sera from two pulmonary carcinoma patients, adenocarcinoma (AD) and small cell carcinoma (SCLC) was screened by immunoblotting using cell lysate of four cell lines (LCN1, large cell neuroendocrine carcinoma (LCNEC); N231, SCLC; A549, AD; RERF-LC-AI, squamous cell carcinoma (SCC)). To identify the antigens recognized by AAs, two-dimensional gel electrophoresis was immunoblotted and target spots were cut out from the membrane and gel. After trypsin digestion, the proteins were analyzed by mass-spectrometry using a liquid chromatography-tandem mass spectrometer. By this method, cytokeratin18 (CK18) and villin1 were identified with AAs in sera from patients with AD and SCLC, respectively. Thus, the expressions of CK18 and villin1 were further immunohistochemically studied on 124 formalin-fixed and paraffin-embedded pulmonary carcinomas of various histologic types (44 AD, 27 SCC, 29 SCLC, and 34 LCNEC) using commercially available CK18 and villin1 antibodies. Positive CK18 immunostaining was observed in almost all cases with staining intensities significantly higher in AD and LCNEC than in SCC and SCLC. Villin1 was detected in 17/44 (38.6%) of AD and 21/34 (61.8%) of LCNEC, respectively, while in only one each of SCLC and SCC. Thus, villin1 and CK18 may be useful markers to distinguish LCNEC/AD from SCLC/SCC, and the present method might be useful to identify specific tumor-associated molecules in sera from pulmonary carcinoma patients with different histologic types.
巻・号 62(3)
ページ 364-73
公開日 2008-12-1
DOI 10.1016/j.lungcan.2008.03.026
PII S0169-5002(08)00186-4
PMID 18485524
MeSH Adenocarcinoma / immunology Adenocarcinoma / pathology Antibodies, Neoplasm / blood* Autoantibodies / blood* Autoantigens / immunology* Blotting, Western Electrophoresis, Gel, Two-Dimensional Humans Immunoblotting Immunoenzyme Techniques Keratin-18 / immunology* Lung Neoplasms / immunology* Lung Neoplasms / pathology Microfilament Proteins / immunology* Small Cell Lung Carcinoma / immunology Small Cell Lung Carcinoma / pathology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tissue Array Analysis Tumor Cells, Cultured
IF 4.702
引用数 34
WOS 分野 RESPIRATORY SYSTEM ONCOLOGY
リソース情報
ヒト・動物細胞