RRC ID 43364
Author Nakachi Y, Yagi K, Nikaido I, Bono H, Tonouchi M, Schönbach C, Okazaki Y.
Title Identification of novel PPARgamma target genes by integrated analysis of ChIP-on-chip and microarray expression data during adipocyte differentiation.
Journal Biochem Biophys Res Commun
Abstract PPARgamma (peroxisome proliferator-activated receptor gamma) acts as a key molecule of adipocyte differentiation, and transactivates multiple target genes involved in lipid metabolic pathways. Identification of PPARgamma target genes will facilitate to predict the extent to which the drugs can affect and also to understand the molecular basis of lipid metabolism. Here, we have identified five target genes regulated directly by PPARgamma during adipocyte differentiation in 3T3-L1 cells using integrated analyses of ChIP-on-chip and expression microarray. We have confirmed the direct PPARgamma regulation of five genes by luciferase reporter assay in NIH-3T3 cells. Of these five genes Hp, Tmem143 and 1100001G20Rik are novel PPARgamma targets. We have also detected PPREs (PPAR response elements) sequences in the promoter region of the five genes computationally. Unexpectedly, most of the PPREs detected proved to be atypical, suggesting the existence of more atypical PPREs than previously thought in the promoter region of PPARgamma regulated genes.
Volume 372(2)
Pages 362-6
Published 2008-7-25
DOI 10.1016/j.bbrc.2008.05.037
PII S0006-291X(08)00929-7
PMID 18489901
MeSH 3T3-L1 Cells Adipocytes / metabolism* Adipogenesis / genetics* Animals Antibodies / immunology Chromatin Immunoprecipitation / methods Gene Expression Regulation, Developmental* Genes, Reporter Luciferases / genetics Mice Oligonucleotide Array Sequence Analysis / methods PPAR gamma / metabolism*
IF 2.985
Times Cited 36
Human and Animal Cells