Reference - Detail
RRC ID | 43384 |
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Author | Nusuetrong P, Pengsuparp T, Meksuriyen D, Tanitsu M, Kikuchi H, Mizugaki M, Shimazu K, Oshima Y, Nakahata N, Yoshida M. |
Title | Satratoxin H generates reactive oxygen species and lipid peroxides in PC12 cells. |
Journal | Biol Pharm Bull |
Abstract |
Satratoxin H, a mycotoxin, is thought to induce apoptosis of PC12 cells through the activation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) in a glutathione (GSH)-sensitive manner. The present study was undertaken to further elucidate the mechanism by which satratoxin H induces cell death in PC12 cells. Satratoxin H caused apoptosis of PC12 cells within 24-h, as determined by DNA fragmentation and flow cytometric analysis. Satratoxin H increased reactive oxygen species (ROS) production and lipid peroxidation, as determined by malondialdehyde formation. These effects were attenuated by incubation of cells with GSH, suggesting that satratoxin H-induced increase in apoptosis of serum-deprived PC12 cells may be partially mediated through the generation of ROS. |
Volume | 31(6) |
Pages | 1115-20 |
Published | 2008-6-1 |
DOI | 10.1248/bpb.31.1115 |
PII | JST.JSTAGE/bpb/31.1115 |
PMID | 18520041 |
MeSH | Animals Antioxidants / pharmacology Cell Survival / drug effects DNA Fragmentation / drug effects Electrophoresis, Agar Gel Flow Cytometry G1 Phase / drug effects Glutathione / pharmacology Indicators and Reagents JNK Mitogen-Activated Protein Kinases / metabolism Lipid Peroxidation / drug effects* PC12 Cells Rats Reactive Oxygen Species / metabolism* Thiobarbituric Acid Reactive Substances / metabolism Trichothecenes / antagonists & inhibitors Trichothecenes / toxicity* p38 Mitogen-Activated Protein Kinases / metabolism |
IF | 1.863 |
Times Cited | 9 |
WOS Category | PHARMACOLOGY & PHARMACY |
Resource | |
Human and Animal Cells | PC-12(RCB0009) |