RRC ID 43388
著者 Kira Y, Nishikawa M.
タイトル The identification and characterization of a new GTP-binding protein (Gbp45) involved in cell proliferation and death related to mitochondrial function.
ジャーナル Cell Mol Biol Lett
Abstract We describe the identification and characterization of a GTP-binding protein with a molecular weight of 45 kD (Gbp45). Gbp45 cDNA was found to overlap with a hypothetical human protein, PTD004, the sequence of which was previously deposited in the databases. The gene for PTD004 was recently found to be one of the ATPases, hOLA1 (human Obg-like ATPase 1). The Gbp45 gene encodes a protein of 396 amino acid residues. Immunocytochemical analysis and examination with GFP-tagged protein revealed that Gbp45 is primarily located in the cytosolic compartment. Immunoblot analysis showed that the Gbp45 protein is strongly expressed in the neuronal tissues and pancreas. T43N and T56N mutations resulted in a loss of Gbp45's ability to bind to GTP and a loss of GTPase activity. In cultured cells, the transfection of wild-type Gbp45 accelerated cell proliferation, though T43N and T56N mutations induced cell death. Down-regulating Gbp45 expression decreased the cell proliferation rate and increased the rate of cell death induced by the inhibition of mitochondrial electron transport. These findings indicate that Gbp45 plays important roles in cell proliferation and death related to mitochondrial function.
巻・号 13(4)
ページ 570-84
公開日 2008-1-1
DOI 10.2478/s11658-008-0023-8
PMID 18536999
PMC PMC6275657
MeSH Amino Acid Sequence Animals Base Sequence Cell Death / physiology* Cell Line Cell Proliferation* Female GTP-Binding Proteins / chemistry GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism* Guanosine Triphosphate / analogs & derivatives Guanosine Triphosphate / metabolism Humans Male Mice Mice, Inbred C57BL Mitochondria / metabolism* Molecular Sequence Data Molecular Weight RNA, Small Interfering / genetics RNA, Small Interfering / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Sequence Alignment Sequence Homology, Amino Acid Tissue Distribution
IF 3.451
引用数 8
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 293(RCB1637)