| RRC ID |
43389
|
| 著者 |
Atsumi N, Ishii G, Kojima M, Sanada M, Fujii S, Ochiai A.
|
| タイトル |
Podoplanin, a novel marker of tumor-initiating cells in human squamous cell carcinoma A431.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Squamous cell carcinoma (SCC) is a malignant tumor that shows morphologic and phenotypic similarities to normally differentiated squamous epithelium. Thus, it may be an ideal model for seeking a marker of tumor-initiating cells (TICs) based on their morphology. Using the human SCC cell line A431, we found that, as a paradigm of cancer stem cells: (1) podoplanin(+) cells generate both podoplanin(+) and podoplanin(-) cells; (2) podoplanin(-) cells rarely generate podoplanin(+) cells; (3) podoplanin(+) cells have higher colony formation efficiency and tumorigenicity than podoplanin(-) cells; (4) localization and morphology of podoplanin(+) cells in a xenografted tumor derived from podoplanin(+) cells are similar with those in human oral SCC tissue or normal epithelium. Furthermore, podoplanin(+) A431 cells share sonic hedgehog and CD44 expression with stem cells in normal squamous epithelium. Hence, we concluded that podoplanin is a novel marker to enrich TICs with stem-cell-like properties from SCC cell line A431.
|
| 巻・号 |
373(1)
|
| ページ |
36-41
|
| 公開日 |
2008-8-15
|
| DOI |
10.1016/j.bbrc.2008.05.163
|
| PII |
S0006-291X(08)01086-3
|
| PMID |
18539139
|
| MeSH |
Animals
Biomarkers, Tumor / analysis*
Carcinoma, Squamous Cell / diagnosis*
Cell Line, Tumor
Hedgehog Proteins / analysis
Humans
Hyaluronan Receptors / analysis
Immunohistochemistry
Membrane Glycoproteins / analysis*
Mice
Mice, SCID
Mouth Mucosa / chemistry
Neoplastic Stem Cells / chemistry*
Transplantation, Heterologous
|
| IF |
2.985
|
| 引用数 |
97
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
A431(RCB0202) |
