論文 - 詳細
| RRC ID | 43394 |
|---|---|
| 著者 | Sugiura S, Edahiro J, Kikuchi K, Sumaru K, Kanamori T. |
| タイトル | Pressure-driven perfusion culture microchamber array for a parallel drug cytotoxicity assay. |
| ジャーナル | Biotechnol Bioeng |
| Abstract |
This article reports a pressure-driven perfusion culture chip developed for parallel drug cytotoxicity assay. The device is composed of an 8 x 5 array of cell culture microchambers with independent perfusion microchannels. It is equipped with a simple interface for convenient access by a micropipette and connection to an external pressure source, which enables easy operation without special training. The unique microchamber structure was carefully designed with consideration of hydrodynamic parameters and was fabricated out of a polydimethylsiloxane by using multilayer photolithography and replica molding. The microchamber structure enables uniform cell loading and perfusion culture without cross-contamination between neighboring microchambers. A parallel cytotoxicity assay was successfully carried out in the 8 x 5 microchamber array to analyze the cytotoxic effects of seven anticancer drugs. The pressure-driven perfusion culture chip, with its simple interface and well-designed microfluidic network, will likely become an advantageous platform for future high-throughput drug screening by microchip. |
| 巻・号 | 100(6) |
| ページ | 1156-65 |
| 公開日 | 2008-8-15 |
| DOI | 10.1002/bit.21836 |
| PMID | 18553395 |
| MeSH | Antineoplastic Agents / administration & dosage Antineoplastic Agents / adverse effects* Biological Assay Cell Count Cell Culture Techniques / instrumentation* Cell Culture Techniques / methods Cell Survival / drug effects Culture Media Diffusion Chambers, Culture / instrumentation Diffusion Chambers, Culture / methods Dimethylpolysiloxanes Equipment Design Equipment Failure Analysis Flow Injection Analysis HeLa Cells Humans Hydrostatic Pressure Microfluidic Analytical Techniques / instrumentation* Microfluidic Analytical Techniques / methods Microfluidics Perfusion* / instrumentation |
| IF | 4.002 |
| 引用数 | 69 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |