論文 - 詳細
| RRC ID | 43403 |
|---|---|
| 著者 | Tatsuta K, Tanaka S, Tajiri T, Shibata S, Komaru A, Ueda Y, Inoue M, Hasegawa M, Suita S, Sueishi K, Taguchi T, Yonemitsu Y. |
| タイトル | Complete elimination of established neuroblastoma by synergistic action of gamma-irradiation and DCs treated with rSeV expressing interferon-beta gene. |
| ジャーナル | Gene Ther |
| Abstract |
Dendritic cell (DC)-based immunotherapy has been investigated as a new therapeutic approach to intractable neuroblastomas; however, only limited clinical effect has been reported. To overcome the relatively low sensitivity of neuroblastomas against immunotherapy, we undertook a preclinical efficacy study to examine murine models to assess the combined effects of gamma-irradiation pretreatment and recombinant Sendai virus (ts-rSeV/dF)-mediated murine interferon-beta (mIFN-beta) gene transfer to DCs using established c1300 neuroblastomas. Similar to intractable neuroblastomas in the clinic, established c1300 tumors were highly resistant to monotherapy with either gamma-irradiation or DCs activated by ts-rSeV/dF without transgene (ts-rSeV/dF-null) that has been shown to be effective against other murine tumors, including B16F10 melanoma. In contrast, immunotherapy using DCs expressing mIFN-beta through ts-rSeV/dF (ts-rSeV/dF-mIFNbeta-DCs) effectively reduced tumor size, and its combination with gamma-irradiation pretreatment dramatically enhanced its antitumor effect, resulting frequently in the complete elimination of established c1300 tumors 7-9 mm in diameter, in a high survival rate among mice, and in the development of protective immunity in the mice against rechallenge by the tumor cells. These results indicate that the combination of ts-rSeV/dF-mIFNbeta-DCs with gamma-irradiation is a hopeful strategy for the treatment of intractable neuroblastomas, warranting further investigation in the clinical setting. |
| 巻・号 | 16(2) |
| ページ | 240-51 |
| 公開日 | 2009-2-1 |
| DOI | 10.1038/gt.2008.161 |
| PII | gt2008161 |
| PMID | 18987675 |
| MeSH | Animals Combined Modality Therapy Dendritic Cells / immunology Dendritic Cells / transplantation* Disease Models, Animal Female Gamma Rays / therapeutic use* Gene Transfer Techniques Genetic Therapy / methods* Genetic Vectors Interferon-beta / biosynthesis Interferon-beta / genetics* Mice Mice, Inbred A Neuroblastoma / immunology Neuroblastoma / pathology Neuroblastoma / radiotherapy Neuroblastoma / therapy* Sendai virus / genetics |
| 引用数 | 10 |
| WOS 分野 | CHEMISTRY, ANALYTICAL BIOCHEMICAL RESEARCH METHODS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C-1300(RCB0283) |