論文 - 詳細
| RRC ID | 43407 |
|---|---|
| 著者 | Noguchi N, Yanagimoto K, Nakaminami H, Wakabayashi M, Iwai N, Wachi M, Sasatsu M. |
| タイトル | Anti-infectious effect of S-benzylisothiourea compound A22, which inhibits the actin-like protein, MreB, in Shigella flexneri. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
S-Benzylisothiourea compound A22 induces coccoid forms in Escherichia coli by inhibiting the function of the actin-like cytoskeletal protein, MreB. The minimum inhibitory concentration of A22 and the minimum concentration to induce coccoid forms for various pathogenic bacteria were determined. At 10 microg/ml, A22 induced coccoid forms in Shigella flexneri but did not inhibit the growth. No alteration of coccoid forms in the Gram-positive bacteria and anaerobic bacteria tested were observed following treatment with A22. To study the relationship between pathogenicity and alterations in bacterial shape, the infectious capacity of A22-induced coccoid S. flexneri was examined using CHO-K1 cells. Invasion of the coccoid cells was significantly reduced, however, no changes in adherence were observed. Using a mutant defective in the type III secretion apparatus, which delivers effectors to the host, we examined the secretion of effectors by A22-induced coccoid S. flexneri. The amount of secreted effectors in the coccoid cells was clearly decreased compared to rod-shaped cells. These results showed that the maintenance of rod-shaped cells by MreB in bacteria was essential for the secretion of effectors via the type III secretion system. Therefore, our results suggest that A22 is a useful lead compound for a novel anti-infectious agent without bactericidal activity and MreB is a candidate target site for development of new anti-infectious agents. |
| 巻・号 | 31(7) |
| ページ | 1327-32 |
| 公開日 | 2008-7-1 |
| DOI | 10.1248/bpb.31.1327 |
| PII | JST.JSTAGE/bpb/31.1327 |
| PMID | 18591769 |
| MeSH | Actins / chemistry Animals Anti-Infective Agents* Bacteria / drug effects Bacteria, Anaerobic / drug effects Bacteria, Anaerobic / ultrastructure CHO Cells Cell Adhesion Cricetinae Cricetulus Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* Electrophoresis, Polyacrylamide Gel Gram-Positive Bacteria / drug effects Gram-Positive Bacteria / ultrastructure Microbial Sensitivity Tests Shigella flexneri / drug effects* Shigella flexneri / pathogenicity Shigella flexneri / ultrastructure Thiourea / analogs & derivatives* Thiourea / pharmacology |
| IF | 1.863 |
| 引用数 | 14 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |