| RRC ID |
43407
|
| 著者 |
Noguchi N, Yanagimoto K, Nakaminami H, Wakabayashi M, Iwai N, Wachi M, Sasatsu M.
|
| タイトル |
Anti-infectious effect of S-benzylisothiourea compound A22, which inhibits the actin-like protein, MreB, in Shigella flexneri.
|
| ジャーナル |
Biol Pharm Bull
|
| Abstract |
S-Benzylisothiourea compound A22 induces coccoid forms in Escherichia coli by inhibiting the function of the actin-like cytoskeletal protein, MreB. The minimum inhibitory concentration of A22 and the minimum concentration to induce coccoid forms for various pathogenic bacteria were determined. At 10 microg/ml, A22 induced coccoid forms in Shigella flexneri but did not inhibit the growth. No alteration of coccoid forms in the Gram-positive bacteria and anaerobic bacteria tested were observed following treatment with A22. To study the relationship between pathogenicity and alterations in bacterial shape, the infectious capacity of A22-induced coccoid S. flexneri was examined using CHO-K1 cells. Invasion of the coccoid cells was significantly reduced, however, no changes in adherence were observed. Using a mutant defective in the type III secretion apparatus, which delivers effectors to the host, we examined the secretion of effectors by A22-induced coccoid S. flexneri. The amount of secreted effectors in the coccoid cells was clearly decreased compared to rod-shaped cells. These results showed that the maintenance of rod-shaped cells by MreB in bacteria was essential for the secretion of effectors via the type III secretion system. Therefore, our results suggest that A22 is a useful lead compound for a novel anti-infectious agent without bactericidal activity and MreB is a candidate target site for development of new anti-infectious agents.
|
| 巻・号 |
31(7)
|
| ページ |
1327-32
|
| 公開日 |
2008-7-1
|
| DOI |
10.1248/bpb.31.1327
|
| PII |
JST.JSTAGE/bpb/31.1327
|
| PMID |
18591769
|
| MeSH |
Actins / chemistry
Animals
Anti-Infective Agents*
Bacteria / drug effects
Bacteria, Anaerobic / drug effects
Bacteria, Anaerobic / ultrastructure
CHO Cells
Cell Adhesion
Cricetinae
Cricetulus
Cytoskeletal Proteins / genetics
Cytoskeletal Proteins / metabolism*
Electrophoresis, Polyacrylamide Gel
Gram-Positive Bacteria / drug effects
Gram-Positive Bacteria / ultrastructure
Microbial Sensitivity Tests
Shigella flexneri / drug effects*
Shigella flexneri / pathogenicity
Shigella flexneri / ultrastructure
Thiourea / analogs & derivatives*
Thiourea / pharmacology
|
| IF |
1.863
|
| 引用数 |
14
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
|
