Reference - Detail
| RRC ID | 43411 |
|---|---|
| Author | Suzuki T, Ishigami Y, Okada N, Kaneko A, Fukutomi R, Isemura M. |
| Title | Differentiation-associated alteration in gene expression of importins and exportins in human leukemia HL-60 cells. |
| Journal | Biomed Res |
| Abstract |
Employing the DNA microarray technique, we previously reported the alteration in gene expression of nucleocytoplasmic transport factors, importins and exportins, induced by 1,25-dihydroxyvitamin D3 (DVD) in human leukemia HL-60 cells. Here, we used the quantitative reverse transcription-polymerase chain reaction method to confirm such previous findings, and compared them with those from the cells treated with all-trans-retinoic acid (ATRA). The results indicated that the gene expression of the transport factors examined was mostly down-regulated following differentiation induced by DVD and ATRA, but importin alpha5 gene expression was up-regulated in either case. The differences were found in the gene expression of importin alpha3 and exportin 6 between the cells after treatments with DVD and ATRA. These variations may be related to the difference between HL-60 cell lineages differentiating into monocytes/macrophages and granulocytes. The present findings provide further evidence to support the important roles of importins and exportins in cell differentiation. |
| Volume | 29(3) |
| Pages | 141-5 |
| Published | 2008-6-1 |
| DOI | 10.2220/biomedres.29.141 |
| PII | JST.JSTAGE/biomedres/29.141 |
| PMID | 18614847 |
| MeSH | Base Sequence Calcitriol / pharmacology DNA Primers Gene Expression* HL-60 Cells Humans Karyopherins / metabolism* Leukemia, Promyelocytic, Acute / genetics Leukemia, Promyelocytic, Acute / metabolism* Leukemia, Promyelocytic, Acute / pathology Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction Tretinoin / pharmacology |
| IF | 1.429 |
| Times Cited | 19 |
| WOS Category | MEDICINE, RESEARCH & EXPERIMENTAL |
| Altmetric score |
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | |