| RRC ID |
43411
|
| Author |
Suzuki T, Ishigami Y, Okada N, Kaneko A, Fukutomi R, Isemura M.
|
| Title |
Differentiation-associated alteration in gene expression of importins and exportins in human leukemia HL-60 cells.
|
| Journal |
Biomed Res
|
| Abstract |
Employing the DNA microarray technique, we previously reported the alteration in gene expression of nucleocytoplasmic transport factors, importins and exportins, induced by 1,25-dihydroxyvitamin D3 (DVD) in human leukemia HL-60 cells. Here, we used the quantitative reverse transcription-polymerase chain reaction method to confirm such previous findings, and compared them with those from the cells treated with all-trans-retinoic acid (ATRA). The results indicated that the gene expression of the transport factors examined was mostly down-regulated following differentiation induced by DVD and ATRA, but importin alpha5 gene expression was up-regulated in either case. The differences were found in the gene expression of importin alpha3 and exportin 6 between the cells after treatments with DVD and ATRA. These variations may be related to the difference between HL-60 cell lineages differentiating into monocytes/macrophages and granulocytes. The present findings provide further evidence to support the important roles of importins and exportins in cell differentiation.
|
| Volume |
29(3)
|
| Pages |
141-5
|
| Published |
2008-6-1
|
| DOI |
10.2220/biomedres.29.141
|
| PII |
JST.JSTAGE/biomedres/29.141
|
| PMID |
18614847
|
| MeSH |
Base Sequence
Calcitriol / pharmacology
DNA Primers
Gene Expression*
HL-60 Cells
Humans
Karyopherins / metabolism*
Leukemia, Promyelocytic, Acute / genetics
Leukemia, Promyelocytic, Acute / metabolism*
Leukemia, Promyelocytic, Acute / pathology
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Tretinoin / pharmacology
|
| IF |
1.429
|
| Times Cited |
19
|
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
|
| Resource |
| Human and Animal Cells |
|
