Reference - Detail
| RRC ID | 43421 |
|---|---|
| Author | Kommineni VK, Nagineni CN, William A, Detrick B, Hooks JJ. |
| Title | IFN-gamma acts as anti-angiogenic cytokine in the human cornea by regulating the expression of VEGF-A and sVEGF-R1. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Inflammatory processes within the cornea are known to be associated with corneal neovascularization (CN). We examined the effects of inflammatory mediators on the expression of angiogenic factors by corneal cells. TNF-alpha and IL-1 induced VEGF-A secretion by corneal fibroblasts (HCRF) and this was inhibited significantly by IFN-gamma. Constitutively secreted VEGF-A by corneal epithelial cells (HCE) was not affected by these cytokines. Moreover, sVEGF-R1(sFlt-1) secretion by HCRF was stimulated significantly by IFN-gamma. JAK-STAT pathway inhibitor reversed the effects of IFN-gamma on VEGF-A and sFlt-1 secretion by HCRF. RT-PCR analysis showed that IFN-gamma influences the expression of VEGF-A and sFlt-1 by affecting their mRNA level. IFN-gamma inhibited TGF-beta induced VEGF-A secretion but not sVEGF-R1 secretion. This is the first report demonstrating the inhibitory and stimulatory effects of IFN-gamma on VEGF-A and sFlt-1 secretion, respectively. Our results suggest that IFN-gamma acts as an anti-angiogenic cytokine in the human cornea. |
| Volume | 374(3) |
| Pages | 479-84 |
| Published | 2008-9-26 |
| DOI | 10.1016/j.bbrc.2008.07.042 |
| PII | S0006-291X(08)01367-3 |
| PMID | 18639520 |
| PMC | PMC2997485 |
| MeSH | Cell Line Cornea / drug effects Cornea / enzymology* Corneal Neovascularization / enzymology* Fibroblasts / drug effects Fibroblasts / enzymology Humans Interferon-gamma / pharmacology Interferon-gamma / physiology* Interleukin-1 / pharmacology Janus Kinase 1 / antagonists & inhibitors RNA, Messenger / biosynthesis Receptor, Fibroblast Growth Factor, Type 1 / agonists* Receptor, Fibroblast Growth Factor, Type 1 / metabolism Transforming Growth Factor beta / pharmacology Vascular Endothelial Growth Factor A / antagonists & inhibitors* Vascular Endothelial Growth Factor A / metabolism |
| IF | 2.985 |
| Times Cited | 26 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | |