論文 - 詳細
| RRC ID | 43444 |
|---|---|
| 著者 | Sekiya M, Hiraishi A, Touyama M, Sakamoto K. |
| タイトル | Oxidative stress induced lipid accumulation via SREBP1c activation in HepG2 cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
SREBP1c (sterol regulatory element-binding protein 1c) is a metabolic-syndrome-associated transcription factor that controls fatty acid biosynthesis under glucose/insulin stimulation. Oxidative stress increases lipid accumulation, which promotes the generation of reactive oxygen species (ROS). However, we know little about the role of oxidative stress in fatty acid biosynthesis. To clarify the action of oxidative stress in lipid accumulation via SREBP1c, we examined SREBP1c activity in H2O2-treated mammalian cells. We introduced a luciferase reporter plasmid carrying the SREBP1c-binding site into HepG2 or COS-7 cells. With increasing H2O2 dose, SREBP1c transcriptional activity increased in HepG2 cells but declined in COS-7 cells. RT-PCR analysis revealed that mRNA expression of SREBP1c gene or of SREBP1c-regulated genes rose H2O2 dose-dependently in HepG2 cells but dropped in COS-7 cells. Lipid accumulation and levels of the nuclear form of SREBP1c increased in H2O2-stimulated HepG2 cells. ROS may stimulate lipid accumulation in HepG2 cells via SREBP1c activation. |
| 巻・号 | 375(4) |
| ページ | 602-7 |
| 公開日 | 2008-10-31 |
| DOI | 10.1016/j.bbrc.2008.08.068 |
| PII | S0006-291X(08)01592-1 |
| PMID | 18727921 |
| MeSH | Animals COS Cells Chlorocebus aethiops Gene Expression Regulation Humans Hydrogen Peroxide / pharmacology Lipid Metabolism* / drug effects Lipid Metabolism* / genetics Obesity / etiology Obesity / genetics Oxidative Stress* / genetics RNA, Messenger / biosynthesis Sterol Regulatory Element Binding Protein 1 / genetics Sterol Regulatory Element Binding Protein 1 / metabolism* Transcription, Genetic Triglycerides / metabolism |
| IF | 2.985 |
| 引用数 | 82 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |