| RRC ID |
43454
|
| 著者 |
Takahashi J, Ishikawa A, Susa T, Kato T, Kato Y.
|
| タイトル |
Cloning and characterization of porcine CArG binding factor A expression in the anterior pituitary.
|
| ジャーナル |
J Reprod Dev
|
| Abstract |
CArG binding factor A (CBF-A) is a transcription factor first isolated from mouse C2 myogenic cells. Several lines of evidence indicate that CBF-A is also present in the anterior pituitary lobe and participates in the process of development and cell transformation. This study was performed to clone porcine CBF-A and to investigate its roles in the porcine anterior pituitary lobe. The predicted amino acid sequence of porcine CBF-A showed a unique insertion of five TG-repeats in the N-terminal region in comparison with those of other mammals, whereas the other regions appeared to be mostly conserved including two RNA recognition motifs in the middle region. Investigation of the expression of CBF-A gene during porcine pituitary development by RT-PCR showed an exclusive and temporary decrease in expression level shortly after birth in both sexes that was gradually but insufficiently restored. The expression of fluorescence protein-fused CBF-A in CHO cells demonstrated that CBF-A is located in the nuclei. We examined whether CBF-A regulates the expression of pituitary hormone genes in CHO cells and found that CBF-A significantly stimulated the promoter activity of growth hormone and prolactin by about 2-fold but did not stimulate the LHbeta gene. The specific DNA binding ability of porcine CBF-A was examined using serial oligonucleotides, CArG box and CC(W)0-6GG (W=A or T). As a result, porcine CBF-A was shown to have a high binding affinity for double- and single-stranded CC(W)6GG but no affinity for the known sequences of the CBF-A-target genes. Accordingly, this study demonstrated that porcine CBF-A may play a role in regulating at least two pituitary hormone genes, GH and PRL.
|
| 巻・号 |
54(6)
|
| ページ |
424-30
|
| 公開日 |
2008-12-1
|
| DOI |
10.1262/jrd.20065
|
| PII |
JST.JSTAGE/jrd/20065
|
| PMID |
18762718
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Binding Sites
Cell Nucleus / chemistry
Cloning, Molecular*
DNA / metabolism
DNA, Complementary / chemistry
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / physiology
Female
Gene Expression*
Gene Expression Regulation
Growth Hormone / genetics
Humans
Male
Mice
Molecular Sequence Data
Pituitary Gland, Anterior / embryology
Pituitary Gland, Anterior / growth & development
Pituitary Gland, Anterior / metabolism*
Prolactin / genetics
Sequence Homology
Swine*
|
| IF |
1.735
|
| 引用数 |
2
|
|
WOS 分野
|
AGRICULTURE, DAIRY & ANIMAL SCIENCE
REPRODUCTIVE BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
CHO-K1(RCB0285) |
