Reference - Detail
| RRC ID | 43458 |
|---|---|
| Author | Yoshikawa Y, Hirayasu H, Tsuzuki S, Fushiki T. |
| Title | Granzyme A causes detachment of alveolar epithelial A549 cells accompanied by promotion of interleukin-8 release. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Granzyme A (GrA) is a serine protease produced in cytotoxic lymphocytes, lung epithelial cells (alveolar type-II cells), and alveolar macrophages. In the present study, recombinant rat GrA (rGrA) was found to cause rounding and detachment of an alveolar type-II epithelial cell line, A549. Also, rGrA stimulated release of a neutrophil chemoattractant, interleukin-8, from the cells, via a mechanism involving microtubule disruption, probably resulting from reduction of cell adhesion to culture dishes. These findings suggest that GrA might be involved in the pathogenesis of certain lung diseases characterized by loss of alveolar wall structures, neutrophil accumulation, and chronic inflammation. |
| Volume | 72(9) |
| Pages | 2481-4 |
| Published | 2008-9-1 |
| DOI | 10.1271/bbb.80362 |
| PII | JST.JSTAGE/bbb/80362 |
| PMID | 18776661 |
| MeSH | Cell Adhesion Cell Line, Tumor Culture Media, Conditioned / chemistry Culture Media, Serum-Free / pharmacology Cytotoxins / pharmacology* Dose-Response Relationship, Drug Epithelial Cells / immunology Epithelial Cells / metabolism* Granzymes / genetics Granzymes / pharmacology* Humans Interleukin-8 / genetics Interleukin-8 / immunology Interleukin-8 / metabolism* Pulmonary Alveoli / cytology Pulmonary Alveoli / immunology Pulmonary Alveoli / metabolism* Recombinant Proteins / pharmacology Time Factors |
| IF | 1.516 |
| Times Cited | 12 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | A549(RCB0098) |