論文 - 詳細
| RRC ID | 43463 |
|---|---|
| 著者 | Lin LJ, Asaoka Y, Tada M, Sanada M, Nannya Y, Tanaka Y, Tateishi K, Ohta M, Seto M, Sasahira N, Tada M, Kawabe T, Zheng CQ, Kanai F, Ogawa S, Omata M. |
| タイトル | Integrated analysis of copy number alterations and loss of heterozygosity in human pancreatic cancer using a high-resolution, single nucleotide polymorphism array. |
| ジャーナル | Oncology |
| Abstract |
OBJECTIVE:To chart molecular genetic events in pancreatic cancer. METHODS:We analyzed genome-wide copy number alterations and loss of heterozygosity (LOH) in 25 established pancreatic cancer cell lines using a high-density single nucleotide polymorphism (SNP) array. We verified the data using genomic PCR and applied them to clinical samples. RESULTS:Twenty-six homozygous deletion regions were detected in at least 1 cell line and LOH was found at 9p, 18q, 17p, 8p, 13q, 6q, 3p, 6p, 22q, 9q and 12q with high frequency (>50%), consistent with a previous study. Moreover, we found 23 amplified regions in at least 2 cell lines, including 8 unreported loci. We then examined representative genes at the 8 amplified loci in matched pairs of pancreatic cancer and normal tissues. The amplification was detected in 1 (7.1%) to 5 (35.7%) of 14 microdissected tissue specimens. CONCLUSION:Using high-resolution SNP arrays, we studied genome-wide copy number alterations and LOH simultaneously. We identified several novel and minute genomic amplifications, which contained candidate oncogenes in human pancreatic cancers. |
| 巻・号 | 75(1-2) |
| ページ | 102-12 |
| 公開日 | 2008-1-1 |
| DOI | 10.1159/000155813 |
| PII | 000155813 |
| PMID | 18787345 |
| MeSH | Cell Line, Tumor Gene Dosage* Humans Loss of Heterozygosity* Pancreatic Neoplasms / genetics* Polymerase Chain Reaction Polymorphism, Single Nucleotide |
| IF | 2.642 |
| 引用数 | 9 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |