| RRC ID |
43538
|
| 著者 |
Islam S, Hassan F, Tumurkhuu G, Dagvadorj J, Koide N, Naiki Y, Yoshida T, Yokochi T.
|
| タイトル |
Receptor activator of nuclear factor-kappa B ligand induces osteoclast formation in RAW 264.7 macrophage cells via augmented production of macrophage-colony-stimulating factor.
|
| ジャーナル |
Microbiol Immunol
|
| Abstract |
RAW 264.7 macrophage cells differentiate into osteoclast-like cells in the presence of RANKL. Participation of M-CSF in RANKL-induced osteoclast formation of RAW 264.7 cells was examined. TRAP-positive osteoclast-like cells appeared in RAW 264.7 cells cultured in the presence of RANKL. RANKL-induced osteoclast formation was markedly inhibited by anti-M-CSF antibody. RANKL augmented M-CSF mRNA expression and M-CSF production in RAW 264.7 cells. Further, anti-M-CSF antibody inhibited the expression of RANK, c-fms, c-fos and TRAP mRNA in RANKL-stimulated RAW 264.7 cells. However, anti-M-CSF antibody did not affect the expression of DC-STAMP in the stimulated cells. Therefore, RANKL was suggested to induce osteoclast formation in RAW 264.7 cells via augmented production of M-CSF. The putative role of M-CSF in RANKL-induced osteoclast formation of RAW 264.7 cells is discussed.
|
| 巻・号 |
52(12)
|
| ページ |
585-90
|
| 公開日 |
2008-12-1
|
| DOI |
10.1111/j.1348-0421.2008.00076.x
|
| PII |
MIM076
|
| PMID |
19120972
|
| MeSH |
Animals
Cell Differentiation*
Cell Line, Transformed
Gene Expression
Macrophage Colony-Stimulating Factor / immunology*
Macrophages / cytology*
Macrophages / immunology
Mice
Osteoclasts / cytology*
Osteoclasts / immunology
RANK Ligand / immunology*
Receptor Activator of Nuclear Factor-kappa B / genetics
Receptor Activator of Nuclear Factor-kappa B / immunology
|
| IF |
1.566
|
| 引用数 |
14
|
|
WOS 分野
|
IMMUNOLOGY
MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
