| RRC ID |
43544
|
| 著者 |
Matsuno T, Hashimoto Y, Adachi S, Omata K, Yoshitaka Y, Ozeki Y, Umezu Y, Tabata Y, Nakamura M, Satoh T.
|
| タイトル |
Preparation of injectable 3D-formed beta-tricalcium phosphate bead/alginate composite for bone tissue engineering.
|
| ジャーナル |
Dent Mater J
|
| Abstract |
A novel, injectable bone tissue engineering material was developed that consisted of beta-tricalcium phosphate (beta-TCP) beads as the solid phase and alginate as the gel phase. To prepare the instantaneously formed composite scaffold, an aqueous calcium chloride solution was dried on the surface of beta-TCP beads and crosslinked with an alginic acid sodium solution, thereby forming stable beta-TCP beads and alginate gel which were injectable via a syringe. This biodegradable composite was a three-dimensional (3D) material that could be used as an injectable scaffold for bone tissue engineering. In particular, the composite with 2.0 wt% alginate concentration exhibited a compressive strength of 69 kPa in dry conditions, which was significantly higher than that exhibited by 1.0 wt%. Furthermore, mesenchymal stem cells (MSC) were 3D-cultured within the composite and then investigated for osteogenic markers. MSC-loaded composite was subjected to scanning electron microscope (SEM) examination and implanted subcutaneously for in vivo experiment. Results showed that the scaffold provided support for osteogenic differentiation. In light of the encouraging results obtained, this novel injectable composite material may be useful for bone tissue engineering.
|
| 巻・号 |
27(6)
|
| ページ |
827-34
|
| 公開日 |
2008-11-1
|
| DOI |
10.4012/dmj.27.827
|
| PMID |
19241692
|
| MeSH |
Absorbable Implants
Alginates / chemistry*
Alkaline Phosphatase / analysis
Animals
Biocompatible Materials / chemistry*
Biomarkers / analysis
Bone Regeneration / physiology*
Calcium Chloride / chemistry
Calcium Phosphates / chemistry*
Cell Culture Techniques
Cell Differentiation / physiology
Compressive Strength
Cross-Linking Reagents / chemistry
Dermatologic Surgical Procedures
Guided Tissue Regeneration / methods*
Humans
Injections
Materials Testing
Mesenchymal Stem Cells / cytology
Mice
Mice, Nude
Microscopy, Electron, Scanning
Osteocalcin / analysis
Osteogenesis / physiology
Stress, Mechanical
Surface Properties
Tissue Engineering / methods*
Tissue Scaffolds / chemistry*
|
| IF |
1.359
|
| 引用数 |
51
|
|
WOS 分野
|
DENTISTRY, ORAL SURGERY & MEDICINE
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
|
