論文 - 詳細
| RRC ID | 43544 |
|---|---|
| 著者 | Matsuno T, Hashimoto Y, Adachi S, Omata K, Yoshitaka Y, Ozeki Y, Umezu Y, Tabata Y, Nakamura M, Satoh T. |
| タイトル | Preparation of injectable 3D-formed beta-tricalcium phosphate bead/alginate composite for bone tissue engineering. |
| ジャーナル | Dent Mater J |
| Abstract |
A novel, injectable bone tissue engineering material was developed that consisted of beta-tricalcium phosphate (beta-TCP) beads as the solid phase and alginate as the gel phase. To prepare the instantaneously formed composite scaffold, an aqueous calcium chloride solution was dried on the surface of beta-TCP beads and crosslinked with an alginic acid sodium solution, thereby forming stable beta-TCP beads and alginate gel which were injectable via a syringe. This biodegradable composite was a three-dimensional (3D) material that could be used as an injectable scaffold for bone tissue engineering. In particular, the composite with 2.0 wt% alginate concentration exhibited a compressive strength of 69 kPa in dry conditions, which was significantly higher than that exhibited by 1.0 wt%. Furthermore, mesenchymal stem cells (MSC) were 3D-cultured within the composite and then investigated for osteogenic markers. MSC-loaded composite was subjected to scanning electron microscope (SEM) examination and implanted subcutaneously for in vivo experiment. Results showed that the scaffold provided support for osteogenic differentiation. In light of the encouraging results obtained, this novel injectable composite material may be useful for bone tissue engineering. |
| 巻・号 | 27(6) |
| ページ | 827-34 |
| 公開日 | 2008-11-1 |
| DOI | 10.4012/dmj.27.827 |
| PMID | 19241692 |
| MeSH | Absorbable Implants Alginates / chemistry* Alkaline Phosphatase / analysis Animals Biocompatible Materials / chemistry* Biomarkers / analysis Bone Regeneration / physiology* Calcium Chloride / chemistry Calcium Phosphates / chemistry* Cell Culture Techniques Cell Differentiation / physiology Compressive Strength Cross-Linking Reagents / chemistry Dermatologic Surgical Procedures Guided Tissue Regeneration / methods* Humans Injections Materials Testing Mesenchymal Stem Cells / cytology Mice Mice, Nude Microscopy, Electron, Scanning Osteocalcin / analysis Osteogenesis / physiology Stress, Mechanical Surface Properties Tissue Engineering / methods* Tissue Scaffolds / chemistry* |
| IF | 1.359 |
| 引用数 | 51 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE MATERIALS SCIENCE, BIOMATERIALS |
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オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |