論文 - 詳細
| RRC ID | 43574 |
|---|---|
| 著者 | Sumitomo T, Nakata M, Yamaguchi M, Terao Y, Kawabata S. |
| タイトル | S-carboxymethylcysteine inhibits adherence of Streptococcus pneumoniae to human alveolar epithelial cells. |
| ジャーナル | J Med Microbiol |
| Abstract |
Streptococcus pneumoniae is a major pathogen of respiratory infections that utilizes platelet-activating factor receptor (PAFR) for firm adherence to host cells. The mucolytic agent S-carboxymethylcysteine (S-CMC) has been shown to exert inhibitory effects against infection by several respiratory pathogens including S. pneumoniae in vitro and in vivo. Moreover, clinical studies have implicated the benefits of S-CMC in preventing exacerbation of chronic obstructive pulmonary disease, which is considered to be related to respiratory infections. In this study, to assess whether the potency of S-CMC is attributable to inhibition of pneumococcal adherence to host cells, an alveolar epithelial cell line stimulated with interleukin-1α was used as a model of inflamed epithelial cells. Despite upregulation of PAFR by inflammatory activation, treatment with S-CMC efficiently inhibited pneumococcal adherence to host epithelial cells. In order to gain insight into the inhibitory mechanism, the effects of S-CMC on PAFR expression were also investigated. Following treatment with S-CMC, PAFR expression was reduced at both mRNA and post-transcriptional levels. Interestingly, S-CMC was also effective in inhibiting pneumococcal adherence to cells transfected with PAFR small interfering RNAs. These results indicate S-CMC as a probable inhibitor targeting numerous epithelial receptors that interact with S. pneumoniae. |
| 巻・号 | 61(Pt 1) |
| ページ | 101-108 |
| 公開日 | 2012-1-1 |
| DOI | 10.1099/jmm.0.033688-0 |
| PMID | 21890513 |
| MeSH | Alveolar Epithelial Cells / microbiology* Anti-Infective Agents, Local / pharmacology* Bacterial Adhesion / drug effects* Carbocysteine / pharmacology* Cell Line, Tumor Epithelial Cells / microbiology Humans Platelet Membrane Glycoproteins / drug effects Platelet Membrane Glycoproteins / metabolism Pulmonary Alveoli / cytology Pulmonary Alveoli / microbiology Receptors, G-Protein-Coupled / drug effects Receptors, G-Protein-Coupled / metabolism Streptococcus pneumoniae / drug effects* Streptococcus pneumoniae / physiology |
| IF | 2.156 |
| 引用数 | 9 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |