RRC ID 43574
著者 Sumitomo T, Nakata M, Yamaguchi M, Terao Y, Kawabata S.
タイトル S-carboxymethylcysteine inhibits adherence of Streptococcus pneumoniae to human alveolar epithelial cells.
ジャーナル J Med Microbiol
Abstract Streptococcus pneumoniae is a major pathogen of respiratory infections that utilizes platelet-activating factor receptor (PAFR) for firm adherence to host cells. The mucolytic agent S-carboxymethylcysteine (S-CMC) has been shown to exert inhibitory effects against infection by several respiratory pathogens including S. pneumoniae in vitro and in vivo. Moreover, clinical studies have implicated the benefits of S-CMC in preventing exacerbation of chronic obstructive pulmonary disease, which is considered to be related to respiratory infections. In this study, to assess whether the potency of S-CMC is attributable to inhibition of pneumococcal adherence to host cells, an alveolar epithelial cell line stimulated with interleukin-1α was used as a model of inflamed epithelial cells. Despite upregulation of PAFR by inflammatory activation, treatment with S-CMC efficiently inhibited pneumococcal adherence to host epithelial cells. In order to gain insight into the inhibitory mechanism, the effects of S-CMC on PAFR expression were also investigated. Following treatment with S-CMC, PAFR expression was reduced at both mRNA and post-transcriptional levels. Interestingly, S-CMC was also effective in inhibiting pneumococcal adherence to cells transfected with PAFR small interfering RNAs. These results indicate S-CMC as a probable inhibitor targeting numerous epithelial receptors that interact with S. pneumoniae.
巻・号 61(Pt 1)
ページ 101-108
公開日 2012-1-1
DOI 10.1099/jmm.0.033688-0
PMID 21890513
MeSH Alveolar Epithelial Cells / microbiology* Anti-Infective Agents, Local / pharmacology* Bacterial Adhesion / drug effects* Carbocysteine / pharmacology* Cell Line, Tumor Epithelial Cells / microbiology Humans Platelet Membrane Glycoproteins / drug effects Platelet Membrane Glycoproteins / metabolism Pulmonary Alveoli / cytology Pulmonary Alveoli / microbiology Receptors, G-Protein-Coupled / drug effects Receptors, G-Protein-Coupled / metabolism Streptococcus pneumoniae / drug effects* Streptococcus pneumoniae / physiology
IF 2.156
引用数 9
WOS 分野 MICROBIOLOGY
リソース情報
ヒト・動物細胞 A549(RCB0098)