論文 - 詳細
| RRC ID | 43576 |
|---|---|
| 著者 | Takano M, Sugano N, Mochizuki S, Koshi RN, Narukawa TS, Sawamoto Y, Ito K. |
| タイトル | Hepatocytes produce tumor necrosis factor-α and interleukin-6 in response to Porphyromonas gingivalis. |
| ジャーナル | J Periodontal Res |
| Abstract |
BACKGROUND AND OBJECTIVE:The liver plays a major role in clearing systemic bacterial infections. In addition, inflammatory cytokines produced in the liver play a critical role in systemic cytokine levels. The aim of this study was to investigate the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) by hepatocytes in response to periodontal pathogens. MATERIAL AND METHODS:The mouse hepatic carcinoma cell line Hepa-1.6 and the mouse macrophage-like cell line RAW 264 were co-cultured in Transwell insert plates. Cells were stimulated with bacterial extracts prepared from Porphyromonas gingivalis and the induction of TNF-α and IL-6 was measured using real-time PCR and ELISA. RESULTS:After stimulation with bacteria, the induction of TNF-α and IL-6 was observed in RAW 264 cells and Hepa-1.6 cells. Significant reduction of TNF-α mRNA expression in Hepa-1.6 cells was observed after treatment with antibody to TNF-α. CONCLUSION:The results obtained in the present study show that P. gingivalis extract induces TNF-α and IL-6 in an in vitro liver model and that macrophage-derived TNF-α mediates the induction of TNF-α in hepatocytes. |
| 巻・号 | 47(1) |
| ページ | 89-94 |
| 公開日 | 2012-2-1 |
| DOI | 10.1111/j.1600-0765.2011.01408.x |
| PMID | 21895661 |
| MeSH | Animals Antibodies / immunology Bacteriological Techniques Cell Line Cell Line, Tumor Coculture Techniques Enzyme-Linked Immunosorbent Assay Hepatocytes / immunology* Immunoglobulin G / immunology Interleukin-6 / immunology* Macrophages / immunology Mice Porphyromonas gingivalis / immunology* Real-Time Polymerase Chain Reaction Time Factors Tumor Necrosis Factor-alpha / immunology* |
| IF | 2.926 |
| 引用数 | 6 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |