RRC ID 43576
Author Takano M, Sugano N, Mochizuki S, Koshi RN, Narukawa TS, Sawamoto Y, Ito K.
Title Hepatocytes produce tumor necrosis factor-α and interleukin-6 in response to Porphyromonas gingivalis.
Journal J Periodontal Res
Abstract BACKGROUND AND OBJECTIVE:The liver plays a major role in clearing systemic bacterial infections. In addition, inflammatory cytokines produced in the liver play a critical role in systemic cytokine levels. The aim of this study was to investigate the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) by hepatocytes in response to periodontal pathogens.
MATERIAL AND METHODS:The mouse hepatic carcinoma cell line Hepa-1.6 and the mouse macrophage-like cell line RAW 264 were co-cultured in Transwell insert plates. Cells were stimulated with bacterial extracts prepared from Porphyromonas gingivalis and the induction of TNF-α and IL-6 was measured using real-time PCR and ELISA.
RESULTS:After stimulation with bacteria, the induction of TNF-α and IL-6 was observed in RAW 264 cells and Hepa-1.6 cells. Significant reduction of TNF-α mRNA expression in Hepa-1.6 cells was observed after treatment with antibody to TNF-α.
CONCLUSION:The results obtained in the present study show that P. gingivalis extract induces TNF-α and IL-6 in an in vitro liver model and that macrophage-derived TNF-α mediates the induction of TNF-α in hepatocytes.
Volume 47(1)
Pages 89-94
Published 2012-2-1
DOI 10.1111/j.1600-0765.2011.01408.x
PMID 21895661
MeSH Animals Antibodies / immunology Bacteriological Techniques Cell Line Cell Line, Tumor Coculture Techniques Enzyme-Linked Immunosorbent Assay Hepatocytes / immunology* Immunoglobulin G / immunology Interleukin-6 / immunology* Macrophages / immunology Mice Porphyromonas gingivalis / immunology* Real-Time Polymerase Chain Reaction Time Factors Tumor Necrosis Factor-alpha / immunology*
IF 2.926
Times Cited 6
Human and Animal Cells RAW 264(RCB0535)