Reference - Detail
| RRC ID | 43579 |
|---|---|
| Author | Takahashi M, Suzuki S, Ishikawa K. |
| Title | Cyclophilin A-EMMPRIN interaction induces invasion of head and neck squamous cell carcinoma. |
| Journal | Oncol Rep |
| Abstract |
Extracellular matrix remodeling crucial to tumorigenesis involves proteolytic enzymes, primarily matrix metalloproteinases (MMPs). MMP production is stimulated by multiple factors, including the extracellular matrix metallo-proteinase inducer EMMPRIN/CD147. Overexpression of EMMPRIN, a member of the immunoglobulin superfamily, promotes invasion, metastasis, growth and survival of malignant cells. Cyclophilin A (CypA) is a multifunctional protein that promotes cancer progression in various cancer types. CypA can interact with and activate EMMPRIN; however, the role of CypA-EMMPRIN interaction in oncogenicity is not completely understood. To investigate tumorigenicity induced by the CypA-EMMPRIN interaction, we stimulated EMMPRIN-expressing head and neck squamous cell carcinoma (HNSCC) cells with CypA. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye assay revealed that HNSCC cell proliferation increased upon stimulation of the cells with CypA, whereas cisplatin-induced cell death decreased after stimulation. Gelatin zymography showed that CypA also induced MMP-9 up-regulation. Moreover, HNSCC cell invasion through Matrigel™-coated membranes was increased upon stimulation of cells with CypA. This elevated invasive potential was abrogated by an EMMPRIN function-blocking antibody. These findings suggest that CypA, through its interaction with EMMPRIN, contributes to HNSCC tumorigenesis. |
| Volume | 27(1) |
| Pages | 198-203 |
| Published | 2012-1-1 |
| DOI | 10.3892/or.2011.1474 |
| PMID | 21956400 |
| MeSH | Basigin / metabolism* Carcinoma, Squamous Cell / metabolism* Carcinoma, Squamous Cell / pathology Cell Line, Tumor Cyclophilin A / metabolism* Head and Neck Neoplasms / metabolism* Head and Neck Neoplasms / pathology Humans Immunoblotting Matrix Metalloproteinase 9 / metabolism Neoplasm Invasiveness |
| IF | 3.417 |
| Times Cited | 15 |
| WOS Category | ONCOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | SAS(RCB1974) |