論文 - 詳細
| RRC ID | 43581 |
|---|---|
| 著者 | Pervin M, Paeng N, Yasui K, Imai S, Isemura M, Yokogoshi H, Nakayama T. |
| タイトル | Effects of Lens culinaris agglutinin on gene expression of gluconeogenic enzymes in the mouse intestine. |
| ジャーナル | J Sci Food Agric |
| Abstract |
BACKGROUND:Lectins are proteins that bind specifically to the carbohydrate moiety of glyco-conjugates. Japanese mistletoe lectin given intragastrically affected cytokine gene expression in the mouse intestine. This study examines the actions of Lens culinaris agglutinin (LCA) on the gene expression of gluconeogenic enzymes in the intestine. RESULTS:The results of quantitative real-time reverse transcription-polymerase chain reaction indicated that LCA caused an up-regulation of the gene expression of glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK). This change was correlated with an increase in the expression of two transcription factors, HNF1α and HNF4α. Experiments using human colonic cancer Caco-2 cells demonstrated that LCA up-regulated the gene expression of G6Pase and PEPCK whereas insulin had the opposite effect. In addition, the observed up-regulation of HNF4α gene expression in the duodenum raises the possibility that the lectin promotes the colorectal cancer. CONCLUSION:Lentil beans should be cooked well to avoid unfavourable effects of LCA. |
| 巻・号 | 92(4) |
| ページ | 857-61 |
| 公開日 | 2012-3-15 |
| DOI | 10.1002/jsfa.4658 |
| PMID | 21969243 |
| MeSH | Animals Caco-2 Cells Colorectal Neoplasms / etiology Colorectal Neoplasms / metabolism Duodenum / enzymology* Duodenum / metabolism Gene Expression Regulation, Enzymologic* Gluconeogenesis* Glucose-6-Phosphatase / genetics Glucose-6-Phosphatase / metabolism Hepatocyte Nuclear Factor 1-alpha / genetics Hepatocyte Nuclear Factor 1-alpha / metabolism Hepatocyte Nuclear Factor 4 / genetics Hepatocyte Nuclear Factor 4 / metabolism Humans Lens Plant / metabolism* Male Mice Mice, Inbred BALB C Neoplasm Proteins / genetics Neoplasm Proteins / metabolism Phosphoenolpyruvate Carboxykinase (ATP) / genetics Phosphoenolpyruvate Carboxykinase (ATP) / metabolism Plant Lectins / adverse effects Plant Lectins / metabolism* RNA, Messenger / metabolism Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction |
| IF | 2.614 |
| 引用数 | 0 |
| WOS 分野 | AGRICULTURE, MULTIDISCIPLINARY CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |