RRC ID 43601
Author Zhu T, Lim BS, Park HC, Son KM, Yang HC.
Title Effects of the iron-chelating agent deferoxamine on triethylene glycol dimethacrylate, 2-hydroxylethyl methacrylate, hydrogen peroxide-induced cytotoxicity.
Journal J Biomed Mater Res B Appl Biomater
Abstract Triethylene glycol dimethacrylate (TEGDMA) and 2-hydroxylethyl methacrylate (HEMA) are known to deplete glutathione in mammalian cells, generate reactive oxygen species (ROS), and cause oxidative stress. In this study, we investigated whether hydroxyl radicals (·OH), the most lethal and genotoxic ROS, and the Fenton reaction are involved in the cytotoxicity of resin monomers to four different cell types, namely MC3T3-E1 preosteoblasts, human dental pulp cells (HDPCs), human gingival fibroblasts, and L929 mouse fibroblasts. Deferoxamine (DFO), an iron chelating agent, effectively protected MC3T3-E1 cells from resin monomer-induced cytotoxicity, indicating that cytotoxicity was caused primarily by hydroxyl radicals. However, DFO only had a protective effect against relatively high concentrations of TEGDMA and HEMA in HDPCs and human gingival fibroblasts, and resin monomer-induced cytotoxicity in L929 was not attenuated by DFO. A labile iron pool (LIP) was detectable only in MC3T3-E1 cells among the four cell types. This indicates that the generation of hydroxyl radicals induced by resin monomers is likely dependent on LIP levels. In contrast to resin monomers, hydrogen peroxide (H(2)O(2))-induced cytotoxicity was not prevented by DFO in any of the cell types examined, although hydroxyl radicals were detected in MC3T3-E1 cells and HDPCs on exposure to exogenous H(2)O(2). This result suggests that generation of hydroxyl radicals is not always the primary cause of cytotoxicity in H(2)O(2)-treated cells.
Volume 100(1)
Pages 197-205
Published 2012-1-1
DOI 10.1002/jbm.b.31939
PMID 22102427
MeSH Animals Cell Line Deferoxamine / chemistry Deferoxamine / pharmacology* Dogs Dose-Response Relationship, Drug Humans Hydrogen Peroxide / chemistry Hydrogen Peroxide / pharmacology* Hydroxyl Radical / metabolism Iron / chemistry Iron / metabolism Materials Testing Methacrylates / chemistry Methacrylates / pharmacology* Mice Oxidants / chemistry Oxidants / pharmacology* Polyethylene Glycols / chemistry Polyethylene Glycols / pharmacology* Polymethacrylic Acids / chemistry Polymethacrylic Acids / pharmacology* Resins, Synthetic / adverse effects Resins, Synthetic / chemistry Resins, Synthetic / pharmacology* Siderophores / chemistry Siderophores / pharmacology*
IF 2.831
Times Cited 9
Human and Animal Cells MC3T3-E1(RCB1126)