論文 - 詳細
| RRC ID | 43616 |
|---|---|
| 著者 | Anno T, Higashi T, Motoyama K, Hirayama F, Uekama K, Arima H. |
| タイトル | Potential use of glucuronylglucosyl-β-cyclodextrin/dendrimer conjugate (G2) as a DNA carrier in vitro and in vivo. |
| ジャーナル | J Drug Target |
| Abstract |
In this study, we evaluated the polyamidoamine starburst dendrimer (dendrimer, generation 2: G2) conjugate with 6-O-α-(4-O-α-D-glucuronyl)-D-glucosyl-β-cyclodextrin (GUG-β-CDE (G2)) as a gene transfer carrier. The in vitro gene transfer activity of GUG-β-CDE (G2, degree of substitution (DS) of cyclodextrin (CyD) 1.8) was remarkably higher than that of dendrimer (G2) conjugate with α-CyD (α-CDE (G2, DS 1.2)) and that with β-CyD(β-CDE (G2, DS 1.3)) in A549 and RAW264.7 cells. The particle size, ζ-potential, DNase I-catalyzed degradation, and cellular association of plasmid DNA (pDNA) complex with GUG-β-CDE (G2, DS 1.8) were almost the same as those of the other CDEs. Fluorescent-labeled GUG-β-CDE (G2, DS 1.8) localized in the nucleus 6 h after transfection of its pDNA complex in A549 cells, suggesting that nuclear localization of pDNA complex with GUG-β-CDE (G2, DS 1.8), at least in part, contributes to its high gene transfer activity. GUG-β-CDE (G2, DS 1.8) provided higher gene transfer activity than α-CDE (G2, DS 1.2) and β-CDE (G2, DS 1.3) in kidney with negligible changes in blood chemistry values 12 h after intravenous injection of pDNA complexes with GUG-β-CDE (G2, DS 1.8) in mice. In conclusion, the present findings suggest that GUG-β-CDE (G2, DS 1.8) has the potential for a novel polymeric pDNA carrier in vitro and in vivo. |
| 巻・号 | 20(3) |
| ページ | 272-80 |
| 公開日 | 2012-4-1 |
| DOI | 10.3109/1061186X.2011.645163 |
| PMID | 22201459 |
| MeSH | Adenocarcinoma / chemistry Adenocarcinoma / metabolism Adenocarcinoma / pathology Animals Biological Transport Cell Line, Transformed Cell Line, Tumor Cell Nucleus / metabolism Cell Nucleus / pathology Chemical Phenomena Dendrimers / adverse effects* Dendrimers / chemistry Dendrimers / pharmacokinetics Female Gene Transfer Techniques* / adverse effects Humans Kidney / chemistry Kidney / metabolism Macrophages / cytology Macrophages / metabolism Mice Mice, Inbred BALB C Nanoparticles / adverse effects* Nanoparticles / chemistry Particle Size Surface Properties Tissue Distribution beta-Cyclodextrins / adverse effects* beta-Cyclodextrins / chemistry beta-Cyclodextrins / pharmacokinetics |
| IF | 3.38 |
| 引用数 | 14 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |