Reference - Detail
|Author||Tamagawa K, Horiuchi T, Wada T, Bannai K, Ando T.|
|Title||Polysaccharide-K (PSK) may suppress surgical stress-induced metastasis in rat colon cancer.|
|Journal||Langenbecks Arch Surg|
PURPOSE:We previously demonstrated that hepatic ischemia and reperfusion (I/R) injury increased liver metastasis and cancer growth of RCN-H4 cells. Using a rat model of hepatic I/R-induced liver metastasis, we investigated the metastasis-suppressing effect of polysaccharide-K (PSK), a biological response modifier composed of protein-bound polysaccharide.
METHODS:Fischer rats underwent 60 min of 70% partial hepatic ischemia. After 60 min of reperfusion, rat colon adenocarcinoma cells (RCN-H4) were inoculated intrasplenically. PSK was administered orally before I/R, after I/R, or before and after I/R. The weights of metastatic lesions of the liver or the numbers of liver metastatic nodules were determined on day 21. The effect of PSK on angiogenesis was studied by a rat cornea model using RCN-H4 cells or a vascular endothelial growth factor (VEGF)-containing pellet and an in vitro VEGF-induced endothelial cell migration assay.
RESULTS:PSK administration significantly (p < 0.05) suppressed the I/R-induced increase in hepatic metastasis of RCN-H4 cells. The suppression of I/R-promoted metastasis was observed irrespective of the timing of administration. Furthermore, PSK significantly suppressed angiogenesis induced by RCN-H4 cells (p < 0.05) and the VEGF pellet (p < 0.01). PSK significantly suppressed the VEGF-induced migration of vascular endothelial cells (p < 0.05).
CONCLUSION:PSK may suppress metastasis induced by hepatic I/R. The suppression of angiogenesis by PSK may be one of the mechanisms of the inhibition of hepatic metastasis.
|MeSH||Animals Cell Line, Tumor Colonic Neoplasms / pathology* Disease Models, Animal Immunologic Factors / therapeutic use* Liver Neoplasms / physiopathology Liver Neoplasms / secondary Male Neovascularization, Physiologic / drug effects* Proteoglycans / therapeutic use* Rats Rats, Inbred F344 Reperfusion Injury / physiopathology Stress, Physiological / drug effects* Stress, Physiological / physiology Vascular Endothelial Growth Factor A / metabolism|
|Human and Animal Cells|