論文 - 詳細
| RRC ID | 43619 |
|---|---|
| 著者 | Yumoto R, Suzuka S, Oda K, Nagai J, Takano M. |
| タイトル | Endocytic uptake of FITC-albumin by human alveolar epithelial cell line A549. |
| ジャーナル | Drug Metab Pharmacokinet |
| Abstract |
The uptake mechanism of FITC-labeled albumin (FITC-albumin) was examined in human alveolar epithelial cell line A549. FITC-albumin uptake by A549 cells was time- and temperature-dependent, and was markedly suppressed at 4°C compared with that at 37°C. The uptake was saturable, and was mediated by a high-affinity, low-capacity system and by a low-affinity, high-capacity system. In the following experiments, we focused on the low-affinity system. FITC-albumin uptake was markedly inhibited by metabolic inhibitors and by a vacuolar H⁺-ATPase, bafilomycin A₁. The uptake was inhibited by clathrin-mediated endocytosis inhibitors (phenylarsine oxide and chlorpromazine). Potassium depletion and hypertonicity that inhibit clathrin-mediated endocytosis also decreased FITC-albumin uptake. On the other hand, caveolae-mediated endocytosis inhibitors (indomethacin and nystatin) did not affect FITC-albumin uptake. In addition, FITC-albumin uptake was inhibited by macropinocytosis inhibitors such as 5-(N-ethyl-N-isopropyl) amiloride. These results suggest that the low-affinity system of FITC-albumin uptake is mediated by endocytosis in A549 cells, predominantly via a clathrin-mediated pathway. Macropinocytosis, but not caveolae-mediated endocytosis, may also be involved. Considering our previous findings, albumin may be transported by a similar mechanism and/or pathway in rat and human alveolar epithelial cells. |
| 巻・号 | 27(3) |
| ページ | 336-43 |
| 公開日 | 2012-1-1 |
| DOI | 10.2133/dmpk.dmpk-11-rg-127 |
| PII | JST.JSTAGE/dmpk/DMPK-11-RG-127 |
| PMID | 22214936 |
| MeSH | Adenosine Triphosphatases / antagonists & inhibitors Adenosine Triphosphatases / metabolism Alveolar Epithelial Cells / drug effects Alveolar Epithelial Cells / metabolism* Caveolae / drug effects Caveolae / metabolism Cell Line Clathrin / antagonists & inhibitors Clathrin / metabolism* Cold Temperature Endocytosis* / drug effects Enzyme Inhibitors / pharmacology Fluorescein-5-isothiocyanate / analogs & derivatives* Fluorescein-5-isothiocyanate / metabolism Humans Hydrogen-Ion Concentration Kinetics Membrane Transport Modulators / pharmacology Osmolar Concentration Pinocytosis / drug effects Potassium / metabolism Serum Albumin / metabolism* Serum Albumin, Bovine / metabolism |
| IF | 2.772 |
| 引用数 | 31 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |