RRC ID |
43650
|
著者 |
Motoyama K, Hayashida K, Higashi T, Arima H.
|
タイトル |
Polypseudorotaxanes of pegylated α-cyclodextrin/polyamidoamine dendrimer conjugate with cyclodextrins as a sustained release system for DNA.
|
ジャーナル |
Bioorg Med Chem
|
Abstract |
Nonviral gene delivery suffers from a number of limitations including short transgene expression times and low transfection efficiency. In this study, we examined whether polypseudorotaxanes (PPRXs) of polyethylene glycol (PEG, molecular weight: 2,000)-grafted α-cyclodextrin (α-CyD)/polyamidoamine dendrimer conjugate (PEG-α-CDE) with CyDs have the potential for the novel sustained release systems for plasmid DNA (pDNA). The PEG-α-CDE/pDNA complex formed PPRXs with α-CyD and γ-CyD solutions, but not with β-CyD solution. In the PEG-α-CDE/CyDs PPRX systems, 20.6mol of α-CyD and 11.8mol of γ-CyD were involved in the PPRXs formation with one PEG chain by α-CyD and γ-CyD, respectively, consistent with in the PEG-dendrimer/CyDs systems. PEG-α-CDE/pDNA/α-CyD PPRX and PEG-α-CDE/pDNA/γ-CyD PPRX formed hexagonal and tetragonal columnar channels in the crystalline phase, respectively. In addition, the CyDs PPRX provided the sustained release of pDNA from PEG-α-CDE complex with pDNA at least 72 h in vitro. The release of pDNA from CyDs PPRX retarded as the volume of dissolution medium decreased. Furthermore, the PEG-α-CDE/γ-CyD PPRX system showed sustained transfection efficiency after intramuscular injection to mice at least for 14days. These results suggest that the PEG-α-CDE/CyD PPRX systems are useful for novel sustained DNA release systems.
|
巻・号 |
20(4)
|
ページ |
1425-33
|
公開日 |
2012-2-15
|
DOI |
10.1016/j.bmc.2011.12.060
|
PII |
S0968-0896(12)00002-8
|
PMID |
22277591
|
MeSH |
Animals
Cell Line, Tumor
Crystallography, X-Ray
DNA / chemistry*
Delayed-Action Preparations / administration & dosage
Delayed-Action Preparations / chemistry*
Delayed-Action Preparations / pharmacology
Dendrimers / chemistry*
Electrophoresis, Gel, Two-Dimensional
Injections, Intramuscular
Male
Mice
Mice, Inbred BALB C
Molecular Structure
Particle Size
Polyamines / chemistry*
Polyamines / pharmacology
Polyethylene Glycols / chemistry
Rotaxanes / chemical synthesis
Rotaxanes / chemistry*
X-Ray Diffraction
alpha-Cyclodextrins / chemistry*
alpha-Cyclodextrins / pharmacology
|
IF |
3.073
|
引用数 |
29
|
WOS 分野
|
CHEMISTRY, MEDICINAL
CHEMISTRY, ORGANIC
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
|