| RRC ID |
43653
|
| 著者 |
Ramalingam M, Young MF, Thomas V, Sun L, Chow LC, Tison CK, Chatterjee K, Miles WC, Simon CG Jr.
|
| タイトル |
Nanofiber scaffold gradients for interfacial tissue engineering.
|
| ジャーナル |
J Biomater Appl
|
| Abstract |
We have designed a 2-spinnerette device that can directly electrospin nanofiber scaffolds containing a gradient in composition that can be used to engineer interfacial tissues such as ligament and tendon. Two types of nanofibers are simultaneously electrospun in an overlapping pattern to create a nonwoven mat of nanofibers containing a composition gradient. The approach is an advance over previous methods due to its versatility - gradients can be formed from any materials that can be electrospun. A dye was used to characterize the 2-spinnerette approach and applicability to tissue engineering was demonstrated by fabricating nanofibers with gradients in amorphous calcium phosphate nanoparticles (nACP). Adhesion and proliferation of osteogenic cells (MC3T3-E1 murine pre-osteoblasts) on gradients was enhanced on the regions of the gradients that contained higher nACP content yielding a graded osteoblast response. Since increases in soluble calcium and phosphate ions stimulate osteoblast function, we measured their release and observed significant release from nanofibers containing nACP. The nanofiber-nACP gradients fabricated herein can be applied to generate tissues with osteoblast gradients such as ligaments or tendons. In conclusion, these results introduce a versatile approach for fabricating nanofiber gradients that can have application for engineering graded tissues.
|
| 巻・号 |
27(6)
|
| ページ |
695-705
|
| 公開日 |
2013-2-1
|
| DOI |
10.1177/0885328211423783
|
| PII |
0885328211423783
|
| PMID |
22286209
|
| PMC |
PMC3508378
|
| MeSH |
3T3 Cells
Animals
Calcium Phosphates / chemistry
Cell Adhesion
Cell Proliferation
Materials Testing
Mice
Microscopy, Electron, Scanning
Nanofibers* / chemistry
Nanofibers* / ultrastructure
Nanoparticles / chemistry
Nanotechnology / instrumentation
Osteoblasts / cytology
Tissue Engineering / instrumentation*
Tissue Scaffolds* / chemistry
|
| IF |
2.22
|
| 引用数 |
41
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
