論文 - 詳細
| RRC ID | 43653 |
|---|---|
| 著者 | Ramalingam M, Young MF, Thomas V, Sun L, Chow LC, Tison CK, Chatterjee K, Miles WC, Simon CG Jr. |
| タイトル | Nanofiber scaffold gradients for interfacial tissue engineering. |
| ジャーナル | J Biomater Appl |
| Abstract |
We have designed a 2-spinnerette device that can directly electrospin nanofiber scaffolds containing a gradient in composition that can be used to engineer interfacial tissues such as ligament and tendon. Two types of nanofibers are simultaneously electrospun in an overlapping pattern to create a nonwoven mat of nanofibers containing a composition gradient. The approach is an advance over previous methods due to its versatility - gradients can be formed from any materials that can be electrospun. A dye was used to characterize the 2-spinnerette approach and applicability to tissue engineering was demonstrated by fabricating nanofibers with gradients in amorphous calcium phosphate nanoparticles (nACP). Adhesion and proliferation of osteogenic cells (MC3T3-E1 murine pre-osteoblasts) on gradients was enhanced on the regions of the gradients that contained higher nACP content yielding a graded osteoblast response. Since increases in soluble calcium and phosphate ions stimulate osteoblast function, we measured their release and observed significant release from nanofibers containing nACP. The nanofiber-nACP gradients fabricated herein can be applied to generate tissues with osteoblast gradients such as ligaments or tendons. In conclusion, these results introduce a versatile approach for fabricating nanofiber gradients that can have application for engineering graded tissues. |
| 巻・号 | 27(6) |
| ページ | 695-705 |
| 公開日 | 2013-2-1 |
| DOI | 10.1177/0885328211423783 |
| PII | 0885328211423783 |
| PMID | 22286209 |
| PMC | PMC3508378 |
| MeSH | 3T3 Cells Animals Calcium Phosphates / chemistry Cell Adhesion Cell Proliferation Materials Testing Mice Microscopy, Electron, Scanning Nanofibers* / chemistry Nanofibers* / ultrastructure Nanoparticles / chemistry Nanotechnology / instrumentation Osteoblasts / cytology Tissue Engineering / instrumentation* Tissue Scaffolds* / chemistry |
| IF | 2.22 |
| 引用数 | 41 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |