| RRC ID |
43683
|
| 著者 |
Ribeiro C, Panadero JA, Sencadas V, Lanceros-Méndez S, Tamaño MN, Moratal D, Salmerón-Sánchez M, Gómez Ribelles JL.
|
| タイトル |
Fibronectin adsorption and cell response on electroactive poly(vinylidene fluoride) films.
|
| ジャーナル |
Biomed Mater
|
| Abstract |
Due to the large potential of electroactive materials in novel tissue engineering strategies, the aim of this work is to determine if the crystalline phase and/or the surface electrical charge of electroactive poly(vinylidene fluoride), PVDF, have influence on the biological response in monolayer cell culture. Non-polar α-PVDF and electroactive β-PVDF were prepared. The β-PVDF films were poled by corona discharge to show negative or positive electrical surface charge density. It has been concluded that hydrophilicity of the PVDF substrates depends significantly on crystalline phase and polarity. Furthermore, by means of atomic force microscopy and an enzyme-linked immunosorbent assay test, it has been shown that positive or negative poling strongly influences the behavior of β-PVDF supports with respect to fibronectin (FN) adsorption, varying the exhibition of adhesion ligands of adsorbed FN. Culture of MC3T3-E1 pre-osteoeblasts proved that cell proliferation depends on surface polarity as well. These results open the viability of cell culture stimulation by mechanical deformation of a piezoelectric substrate that results in varying electrical charge densities on the substrate surface.
|
| 巻・号 |
7(3)
|
| ページ |
035004
|
| 公開日 |
2012-6-1
|
| DOI |
10.1088/1748-6041/7/3/035004
|
| PMID |
22356773
|
| MeSH |
3T3 Cells
Adsorption
Animals
Cell Adhesion / drug effects*
Cell Survival / drug effects*
Coated Materials, Biocompatible / chemistry
Coated Materials, Biocompatible / pharmacology*
Fibronectins / chemistry
Fibronectins / pharmacology*
Materials Testing
Membranes, Artificial*
Mice
Polyvinyls / chemistry*
Static Electricity
Surface Properties
|
| IF |
3.174
|
| 引用数 |
46
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
