論文 - 詳細
| RRC ID | 43693 |
|---|---|
| 著者 | Oyane A, Yazaki Y, Araki H, Sogo Y, Ito A, Yamazaki A, Tsurushima H. |
| タイトル | Fabrication of a DNA-lipid-apatite composite layer for efficient and area-specific gene transfer. |
| ジャーナル | J Mater Sci Mater Med |
| Abstract |
A surface-mediated gene transfer system using biocompatible apatite-based composite layers has great potential for tissue engineering. Among the apatite-based composite layers developed to date, we focused on a DNA-lipid-apatite composite layer (DLp-Ap layer), which has the advantage of relatively high efficiency as a non-viral system. In this study, various lipid transfection reagents, including a newly developed reagent, polyamidoamine dendron-bearing lipid (PD), were employed to prepare the DLp-Ap layer, and the preparation condition was optimized in terms of efficiency of gene transfer to epithelial-like CHO-K1 cells in the presence of serum. The optimized DLp-Ap layer derived from PD had the highest gene transfer efficiency among all the apatite-based composite layers prepared in this study. In addition, the optimized DLp-Ap layer demonstrated higher gene transfer efficiency in the presence of serum than the conventional particle-mediated systems using commercially available lipid transfection reagents. It was also shown that the optimized DLp-Ap layer mediated the area-specific gene transfer on its surface, i.e., DNA was preferentially transferred to the cells adhering to the surface of the layer. The present gene transfer system using the PD-derived DLp-Ap layer, with the advantages of high efficiency in the presence of serum and area-specificity, would be useful in tissue engineering. |
| 巻・号 | 23(4) |
| ページ | 1011-9 |
| 公開日 | 2012-4-1 |
| DOI | 10.1007/s10856-012-4581-y |
| PMID | 22367107 |
| MeSH | Animals Apatites / chemistry* CHO Cells Cricetinae DNA / chemistry* Gene Transfer Techniques* Lipids / chemistry* Microscopy, Electron, Scanning Spectrophotometry, Ultraviolet X-Ray Diffraction |
| IF | 2.489 |
| 引用数 | 9 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1(RCB0285) |