論文 - 詳細
| RRC ID | 43698 |
|---|---|
| 著者 | Zhao WH, Hu ZQ. |
| タイトル | Up-regulation of IL-33 expression in various types of murine cells by IL-3 and IL-4. |
| ジャーナル | Cytokine |
| Abstract |
The crucial roles of the novel cytokine IL-33 in allergic, inflammatory, infectious and autoimmune diseases are becoming characterized. However, the cytokines which regulate IL-33 expression and secretion are still largely unknown. In this study, IL-3 and IL-4 were found to up-regulate IL-33 mRNA expression in mouse peritoneal exudate cells by a two-color DNA microarray and further confirmed by real time PCR and ELISA. IL-3 and IL-4 synergistically promote IL-33 mRNA expression and IL-33 intracrine in the heterogeneous cell populations as peritoneal exudates cells, bone marrow cells and splenic cells. IL-3 and IL-4 also induced IL-33 introcrine in the peritoneal exudate cells from the macrophage-deficient op/op mice, suggesting that macrophage is not the only target of IL-3 and IL-4 in the heterogeneous peritoneal exudate cells. Furthermore, IL-3 and IL-4 were verified to promote the IL-33 intracrine in the homogeneous cell population as fibroblasts and mast cells. These results indicate that up-regulation of IL-33 expression by IL-3 and IL-4 is not a feature particular to a specific type of cells. Up to 100 cytokines were screened, but none of them stimulated the secretion or release of IL-33 in the culture system. In summary, we confirm for the first time that IL-3 and IL-4 are critical for IL-33 intracrine in murine cells of various types, indicating that IL-3 and IL-4 may play an important role in the constitutive expression of IL-33 in vivo. |
| 巻・号 | 58(2) |
| ページ | 267-73 |
| 公開日 | 2012-5-1 |
| DOI | 10.1016/j.cyto.2012.01.019 |
| PII | S1043-4666(12)00051-8 |
| PMID | 22370606 |
| MeSH | Animals Base Sequence DNA Primers Enzyme-Linked Immunosorbent Assay Female Interleukin-3 / physiology* Interleukin-33 Interleukin-4 / physiology* Interleukins / metabolism* Ionomycin / pharmacology Lipopolysaccharides / pharmacology Mice Mice, Inbred C57BL Oligonucleotide Array Sequence Analysis Real-Time Polymerase Chain Reaction Tetradecanoylphorbol Acetate / pharmacology Up-Regulation* |
| IF | 2.952 |
| 引用数 | 15 |
| WOS 分野 | IMMUNOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |