Reference - Detail
|Author||Nishizawa H, Handayaningsih AE, Iguchi G, Cho Y, Takahashi M, Yamamoto M, Suda K, Kasahara K, Hakuno F, Yamanouchi K, Nishihara M, Seino S, Takahashi S, Takahashi Y.|
|Title||Enhanced oxidative stress in GH-transgenic rat and acromegaly in humans.|
|Journal||Growth Horm IGF Res|
BACKGROUND:Excessive oxidative stress plays a causal role in various diseases such as diabetes, hypertension, atherosclerosis, and heart failure. Acromegaly is a pathological condition associated with excess growth hormone (GH) and insulin-like growth factor-I (IGF-I) and a high prevalence of diabetes, hypertension, atherosclerosis, and heart failure; resulting in premature death. We hypothesized that these conditions may be associated with increased oxidative stress.
OBJECTIVE AND METHODS:We explored the oxidative stress levels in the serum and tissues of GH-transgenic rats as an animal model for acromegaly. We also measured the oxidative stress levels in the serum of patients with acromegaly and age-, sex-, and BMI-matched control subjects. We examined the effects of GH and IGF-I on reactive oxygen species (ROS) production in C2C12 myocytes.
RESULTS:The levels of an oxidative stress marker, serum thiobarbituric acid reactive substances (TBARS) were increased in the GH-transgenic rats. Further, tissue oxidative stress damage was enhanced in the cardiomyocytes and vascular smooth muscle cells in the aorta of the GH-transgenic rats. In addition, serum TBARS levels and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels were increased in acromegaly in humans. IGF-I but not GH induced ROS production in C2C12 myocytes in vitro.
CONCLUSIONS:These data indicate that the increased levels of IGF-I are associated with enhanced oxidative stress in rats and humans. In addition, increased ROS may play an important role in the complications and premature death in acromegaly.
|MeSH||Acromegaly / metabolism* Animals Animals, Genetically Modified Dose-Response Relationship, Drug Female Growth Hormone / genetics Growth Hormone / metabolism* Humans Insulin-Like Growth Factor I / metabolism Male Mice Muscle Cells / cytology Oxidative Stress* Oxygen / chemistry Prevalence Rats Reactive Oxygen Species|
|WOS Category||ENDOCRINOLOGY & METABOLISM CELL BIOLOGY|
|Human and Animal Cells|