RRC ID 43714
Author Zhang Z, Zhang J, Miao L, Liu K, Yang S, Pan C, Jiao B.
Title Interleukin-11 promotes the progress of gastric carcinoma via abnormally expressed versican.
Journal Int J Biol Sci
Abstract Versican, a ubiquitous component of the extracellular matrix (ECM), accumulates both in tumor stroma and cancer cells and is highly regulated by various cytokines. The aberrant expression of versican and its isoforms is known to modulate cell proliferation, differentiation, and migration, all of which are features of the invasion and metastasis of cancer; versican is also known to favour the homeostasis of the ECM. Interleukin-11 (IL-11) is an important cytokine that exhibits a wide variety of biological effects in gastric cancer development. Here, we analysed the expression of versican isoforms and found that the major isoforms expressed by both gastric carcinoma tissue and gastric cell lines were V0 and V1, and V1 was significantly higher in gastric carcinoma tissue. The treatment of the gastric cell lines AGS and MKN45 with rhIL-11 resulted in a significant increase in the expression of V0 and V1. Exogenous IL-11 increased migration in AGS and MKN45 cells, whereas these effects were reversed when the expression of V0 and V1 were abolished by siRNA targeting versican V0/V1. Collectively, these findings suggest that the abnormally expressed versican and its isoforms participate, at least in part, in the progress of gastric carcinoma triggered by IL-11.
Volume 8(3)
Pages 383-93
Published 2012-1-1
DOI 10.7150/ijbs.3579
PII ijbsv08p0383
PMID 22393310
PMC PMC3291855
MeSH Adenocarcinoma / genetics* Adenocarcinoma / metabolism Adenocarcinoma / pathology Aged Cell Division / drug effects Cell Line, Tumor / drug effects Cell Line, Tumor / metabolism Cell Movement / drug effects Cells, Cultured / drug effects Cells, Cultured / metabolism Disease Progression Extracellular Matrix / physiology Female Gastric Mucosa / metabolism Gene Expression Regulation, Neoplastic / drug effects* Humans Interleukin-11 / pharmacology* Interleukin-11 / physiology Male Middle Aged Neoplasm Proteins / antagonists & inhibitors Neoplasm Proteins / biosynthesis Neoplasm Proteins / chemistry Neoplasm Proteins / genetics Neoplasm Proteins / physiology* Protein Isoforms / biosynthesis Protein Isoforms / chemistry Protein Isoforms / genetics Protein Isoforms / physiology RNA Interference RNA, Messenger / biosynthesis RNA, Messenger / genetics RNA, Neoplasm / biosynthesis RNA, Neoplasm / genetics RNA, Small Interfering / pharmacology Recombinant Proteins / pharmacology Reverse Transcriptase Polymerase Chain Reaction Stomach / cytology Stomach Neoplasms / genetics* Stomach Neoplasms / metabolism Stomach Neoplasms / pathology Versicans / antagonists & inhibitors Versicans / biosynthesis Versicans / chemistry Versicans / genetics Versicans / physiology*
IF 4.858
Times Cited 23
Human and Animal Cells MKN45(RCB1001) SH-10-TC(RCB1940) HGC-27(RCB0500)