論文 - 詳細
| RRC ID | 43717 |
|---|---|
| 著者 | Yue XS, Fujishiro M, Nishioka C, Arai T, Takahashi E, Gong JS, Akaike T, Ito Y. |
| タイトル | Feeder cells support the culture of induced pluripotent stem cells even after chemical fixation. |
| ジャーナル | PLoS One |
| Abstract |
Chemically fixed mouse embryonic fibroblasts (MEFs), instead of live feeder cells, were applied to the maintenance of mouse induced pluripotent stem (miPS) cells. Formaldehyde and glutaraldehyde were used for chemical fixation. The chemically fixed MEF feeders maintained the pluripotency of miPS cells, as well as their undifferentiated state. Furthermore, the chemically fixed MEF feeders were reused several times without affecting their functions. These results indicate that chemical fixation can be applied to modify biological feeders chemically, without losing their original functions. Chemically fixed MEF feeders will be applicable to other stem cell cultures as a reusable extracellular matrix candidate that can be preserved on a long-term basis. |
| 巻・号 | 7(3) |
| ページ | e32707 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0032707 |
| PII | PONE-D-11-19849 |
| PMID | 22396791 |
| PMC | PMC3292564 |
| MeSH | Alkaline Phosphatase / metabolism Animals Cell Culture Techniques Cell Differentiation Cell Separation Coculture Techniques Extracellular Matrix / metabolism Feeder Cells / cytology* Fibroblasts / cytology* Flow Cytometry Green Fluorescent Proteins / metabolism Homeodomain Proteins / metabolism Induced Pluripotent Stem Cells / cytology* Mice Microscopy, Fluorescence / methods Nanog Homeobox Protein Neurons / metabolism Stem Cells / cytology |
| IF | 2.74 |
| 引用数 | 24 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | miPS |