| RRC ID |
43717
|
| 著者 |
Yue XS, Fujishiro M, Nishioka C, Arai T, Takahashi E, Gong JS, Akaike T, Ito Y.
|
| タイトル |
Feeder cells support the culture of induced pluripotent stem cells even after chemical fixation.
|
| ジャーナル |
PLoS One
|
| Abstract |
Chemically fixed mouse embryonic fibroblasts (MEFs), instead of live feeder cells, were applied to the maintenance of mouse induced pluripotent stem (miPS) cells. Formaldehyde and glutaraldehyde were used for chemical fixation. The chemically fixed MEF feeders maintained the pluripotency of miPS cells, as well as their undifferentiated state. Furthermore, the chemically fixed MEF feeders were reused several times without affecting their functions. These results indicate that chemical fixation can be applied to modify biological feeders chemically, without losing their original functions. Chemically fixed MEF feeders will be applicable to other stem cell cultures as a reusable extracellular matrix candidate that can be preserved on a long-term basis.
|
| 巻・号 |
7(3)
|
| ページ |
e32707
|
| 公開日 |
2012-1-1
|
| DOI |
10.1371/journal.pone.0032707
|
| PII |
PONE-D-11-19849
|
| PMID |
22396791
|
| PMC |
PMC3292564
|
| MeSH |
Alkaline Phosphatase / metabolism
Animals
Cell Culture Techniques
Cell Differentiation
Cell Separation
Coculture Techniques
Extracellular Matrix / metabolism
Feeder Cells / cytology*
Fibroblasts / cytology*
Flow Cytometry
Green Fluorescent Proteins / metabolism
Homeodomain Proteins / metabolism
Induced Pluripotent Stem Cells / cytology*
Mice
Microscopy, Fluorescence / methods
Nanog Homeobox Protein
Neurons / metabolism
Stem Cells / cytology
|
| IF |
2.74
|
| 引用数 |
24
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
miPS |
