| RRC ID |
43746
|
| 著者 |
Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.
|
| タイトル |
Insulin-like growth factor I receptor involvement in proliferation of NOR-P1 cells in serum-free media.
|
| ジャーナル |
J Cell Biochem
|
| Abstract |
Insulin-like growth factor (IGF)-I is up-regulated in pancreatic cancer tissues. Pancreatic cancer cell lines were analyzed in serum-free media as a model of the fibrous tissues that these cells often invade. Pancreatic cancer surgical specimens were immunostained with anti-IGF-I receptor (IGF-IR)β antibody. The growth of pancreatic cancer cells in serum-free media was also analyzed. Cell lysates were analyzed for protein by western blot analysis. Cells cultured in the presence of picropodophyllin (PPP), LY294002, or PD98059, were subjected to cell proliferation and scratch assays. In addition, BrdU uptake and apoptosis were analyzed in these cells. IGF-IRβ was detected in pancreatic cancer cells invading fibrous tissues. NOR-P1 grew most rapidly in serum-free media. The concentrations of IGF-I and IGF-II in the media were higher in NOR-P1 than the other cell lines. Cell proliferation in NOR-P1 cells was enhanced by IGF-I or IGF-II treatment more than in MIA-Paca2 or PK-1 cells. PPP, LY294002, and PD98059 suppressed proliferation and motility of NOR-P1 cells and inhibited BrdU uptake, while PPP induced apoptosis. IGF-IRβ may be a potential therapeutic target to inhibit invasion of pancreatic cancer.
|
| 巻・号 |
113(8)
|
| ページ |
2714-20
|
| 公開日 |
2012-8-1
|
| DOI |
10.1002/jcb.24149
|
| PMID |
22441995
|
| MeSH |
Apoptosis / drug effects*
Cell Line, Tumor
Cell Proliferation / drug effects*
Culture Media, Serum-Free
Deoxyuridine / pharmacology
Humans
Insulin-Like Growth Factor I / metabolism
Insulin-Like Growth Factor I / pharmacology
Insulin-Like Growth Factor II / metabolism
Insulin-Like Growth Factor II / pharmacology
Receptor, IGF Type 1 / metabolism*
|
| IF |
4.237
|
| 引用数 |
6
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
