論文 - 詳細
| RRC ID | 43752 |
|---|---|
| 著者 | Yoshida E, Yoshimura Y, Uo M, Yoshinari M, Hayakawa T. |
| タイトル | Influence of nanometer smoothness and fibronectin immobilization of titanium surface on MC3T3-E1 cell behavior. |
| ジャーナル | J Biomed Mater Res A |
| Abstract |
The aim of the present study was to evaluate the influence of mechanical treatment, namely, nanometer smoothing (Ra: approximately 2.0 nm) and sandblasting (Ra: approximately 1.0 μm), as well as biochemical treatment, namely, fibronectin immobilization, of a titanium surface on osteoblast-like cell behavior. Cell proliferation was monitored by measurements of DNA content and ALP activity; osteocalcin production and mineralization behavior were also evaluated, in addition to morphological observation of attached cells. Fibronectin could be immobilized by the tresyl chloride-activation method. A sandblasted surface resulted in significantly more DNA than a nanometer-smooth surface, but fibronectin immobilization did not result in a significant increase of DNA at 52 days of cell culture. The nanometer-smooth surface showed highest ALP activity and osteocalcin production. FN immobilization decreased ALP activity for the nanometer-smooth surface, but increased it for the sandblasted surface. The nanometer-smooth surface also showed the highest osteocalcin production. Scanning electron microscopy showed interesting phenomena of the attached cells. Attached cell area was more rapidly increased on the nanometer-smooth surface than on the sandblasted surface. It was suggested that cultured cells on the nanometer-smooth surface began to spread earlier and that the proportion of spreading cells among total attached cells increased sooner on the nanometer-smooth surface than on the sandblasted rough surface. It appeared that FN immobilization influenced the arrangement of attached cells. In conclusion, the nanometer-smooth surface employed in the present study was beneficial for the differentiation of MC3T3-E1 cells. FN immobilization influenced the morphologies of attached cells. |
| 巻・号 | 100(6) |
| ページ | 1556-64 |
| 公開日 | 2012-6-1 |
| DOI | 10.1002/jbm.a.34084 |
| PMID | 22447768 |
| MeSH | Alkaline Phosphatase / metabolism Animals Biocompatible Materials / chemistry Biocompatible Materials / metabolism* Cell Differentiation Cell Line Cell Proliferation Dental Implants* Fibronectins / chemistry Fibronectins / metabolism* Immobilized Proteins / chemistry Immobilized Proteins / metabolism Mice Osteoblasts / cytology* Osteoblasts / metabolism Osteocalcin / metabolism Surface Properties Titanium / chemistry Titanium / metabolism* |
| IF | 3.525 |
| 引用数 | 16 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |