RRC ID 43753
著者 Amemiya Y, Kawano K, Matsusaki M, Akashi M, Nakamura N, Nakamura C.
タイトル Formation of nanofilms on cell surfaces to improve the insertion efficiency of a nanoneedle into cells.
ジャーナル Biochem Biophys Res Commun
Abstract A nanoneedle, an atomic force microscope (AFM) tip etched to 200 nm in diameter and 10 μm in length, can be inserted into cells with the aid of an AFM and has been used to introduce functional molecules into cells and to analyze intracellular information with minimal cell damage. However, some cell lines have shown low insertion efficiency of the nanoneedle. Improvement in the insertion efficiency of a nanoneedle into such cells is a significant issue for nanoneedle-based cell manipulation and analysis. Here, we have formed nanofilms composed of extracellular matrix molecules on cell surfaces and found that the formation of the nanofilms improved insertion efficiency of a nanoneedle into fibroblast and neural cells. The nanofilms were shown to improve insertion efficiency even in cells in which the formation of actin stress fibers was inhibited by the ROCK inhibitor Y27632, suggesting that the nanofilms with the mesh structure directly contributed to the improved insertion efficiency of a nanoneedle.
巻・号 420(3)
ページ 662-5
公開日 2012-4-13
DOI 10.1016/j.bbrc.2012.03.062
PII S0006-291X(12)00509-8
PMID 22450311
MeSH Amides / pharmacology Animals BALB 3T3 Cells Cell Membrane / chemistry* Cells / chemistry* Enzyme Inhibitors / pharmacology Extracellular Matrix Proteins / chemistry Mice Microscopy, Atomic Force Nanostructures / chemistry* Neurons / chemistry Pyridines / pharmacology Stress Fibers / chemistry Stress Fibers / drug effects Stress Fibers / enzymology rho-Associated Kinases / antagonists & inhibitors
IF 2.985
引用数 6
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞