論文 - 詳細
| RRC ID | 43770 |
|---|---|
| 著者 | Kwak EJ, Lee YS, Choi EM. |
| タイトル | Effect of magnolol on the function of osteoblastic MC3T3-E1 cells. |
| ジャーナル | Mediators Inflamm |
| Abstract |
OBJECTIVES:In the present study, the ability of magnolol, a hydroxylated biphenyl compound isolated from Magnolia officinalis, to stimulate osteoblast function and inhibit the release of bone-resorbing mediators was investigated in osteoblastic MC3T3-E1 cells. METHODS:Osteoblast function was measured by cell growth, alkaline phosphatase activity, collagen synthesis, and mineralization. Glutathione content was also measured in the cells. Bone-resorbing cytokines, receptor activator of nuclear factor-κB ligand (RANKL), TNF-α, and IL-6 were measured with an enzyme immunoassay system. RESULTS:Magnolol caused a significant elevation of cell growth, alkaline phosphatase activity, collagen synthesis, mineralization, and glutathione content in the cells (P < 0.05). Skeletal turnover is orchestrated by a complex network of regulatory factors. Among cytokines, RANKL, TNF-α, and IL-6 were found to be key osteoclastogenetic molecules produced by osteoblasts. Magnolol significantly (P < 0.05) decreased the production of osteoclast differentiation inducing factors such as RANKL, TNF-α, and IL-6 in the presence of antimycin A, which inhibits mitochondrial electron transport and has been used as an ROS generator. CONCLUSION:Magnolol might be a candidate as an agent for the prevention of bone disorders such as osteoporosis. |
| 巻・号 | 2012 |
| ページ | 829650 |
| 公開日 | 2012-1-1 |
| DOI | 10.1155/2012/829650 |
| PMID | 22474400 |
| PMC | PMC3306956 |
| MeSH | Alkaline Phosphatase / metabolism Animals Antimycin A / pharmacology Biphenyl Compounds / pharmacology* Cell Line Collagen / metabolism Cytokines / metabolism Glutathione / metabolism Interleukin-6 / metabolism Lignans / pharmacology* Mice Osteoblasts / drug effects* Osteoblasts / metabolism* RANK Ligand / metabolism Tumor Necrosis Factor-alpha / metabolism |
| IF | 3.758 |
| 引用数 | 14 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |