RRC ID 43773
Author Arung ET, Kuspradini H, Kusuma IW, Shimizu K, Kondo R.
Title Validation of Eupatorium triplinerve Vahl leaves, a skin care herb from East Kalimantan, using a melanin biosynthesis assay.
Journal J Acupunct Meridian Stud
Abstract In searching for a new material made from natural resources that could be used as a whitening agent, we focused on the plants used for skin treatment by the native people of East Kalimantan. The methanol extract of the leaves of Eupatorium triplinerve Vahl showed antimelanogenesis activity in a melanin biosynthesis assay. By activity-guided fractionation, 7-methoxycoumarin (1) was isolated as an active compound. The IC50 of 1 on mushroom tyrosinase was 2360 μM (L-tyrosine was used as the substrate) and above 2840 μM (L-DOPA was used as the substrate), respectively. Regarding melanin formation inhibition in B16 melanoma cells, the IC50 of 1 was 1780 μM with 83% cell viability at IC50. Based on these results, we validated that the leaf extract is in line with the traditional use of the Dayak tribe in East Kalimantan.
Volume 5(2)
Pages 87-92
Published 2012-4
DOI 10.1016/j.jams.2012.01.003
PII S2005-2901(12)00019-2
PMID 22483187
MeSH Bleaching Agents / pharmacology* Borneo Cell Line, Tumor Cell Survival / drug effects Coumarins / isolation & purification Coumarins / pharmacology* Eupatorium / chemistry* Humans Inhibitory Concentration 50 Levodopa / antagonists & inhibitors Melanins / biosynthesis* Melanoma, Experimental Monophenol Monooxygenase / antagonists & inhibitors Plant Extracts / chemistry Plant Extracts / pharmacology* Plant Leaves Skin Care
Human and Animal Cells