| RRC ID |
43773
|
| 著者 |
Arung ET, Kuspradini H, Kusuma IW, Shimizu K, Kondo R.
|
| タイトル |
Validation of Eupatorium triplinerve Vahl leaves, a skin care herb from East Kalimantan, using a melanin biosynthesis assay.
|
| ジャーナル |
J Acupunct Meridian Stud
|
| Abstract |
In searching for a new material made from natural resources that could be used as a whitening agent, we focused on the plants used for skin treatment by the native people of East Kalimantan. The methanol extract of the leaves of Eupatorium triplinerve Vahl showed antimelanogenesis activity in a melanin biosynthesis assay. By activity-guided fractionation, 7-methoxycoumarin (1) was isolated as an active compound. The IC50 of 1 on mushroom tyrosinase was 2360 μM (L-tyrosine was used as the substrate) and above 2840 μM (L-DOPA was used as the substrate), respectively. Regarding melanin formation inhibition in B16 melanoma cells, the IC50 of 1 was 1780 μM with 83% cell viability at IC50. Based on these results, we validated that the leaf extract is in line with the traditional use of the Dayak tribe in East Kalimantan.
|
| 巻・号 |
5(2)
|
| ページ |
87-92
|
| 公開日 |
2012-4-1
|
| DOI |
10.1016/j.jams.2012.01.003
|
| PII |
S2005-2901(12)00019-2
|
| PMID |
22483187
|
| MeSH |
Bleaching Agents / pharmacology*
Borneo
Cell Line, Tumor
Cell Survival / drug effects
Coumarins / isolation & purification
Coumarins / pharmacology*
Eupatorium / chemistry*
Humans
Inhibitory Concentration 50
Levodopa / antagonists & inhibitors
Melanins / biosynthesis*
Melanoma, Experimental
Monophenol Monooxygenase / antagonists & inhibitors
Plant Extracts / chemistry
Plant Extracts / pharmacology*
Plant Leaves
Skin Care
|
| 引用数 |
6
|
| リソース情報 |
| ヒト・動物細胞 |
B16 melanoma |
