RRC ID 43782
Author Ito J, Hashimoto T, Nakamura S, Aita Y, Yamazaki T, Schlegel W, Takimoto K, Maturana AD.
Title Splicing transitions of the anchoring protein ENH during striated muscle development.
Journal Biochem Biophys Res Commun
Abstract The ENH (PDLIM5) protein acts as a scaffold to tether various functional proteins at subcellular sites via PDZ and three LIM domains. Splicing of the ENH primary transcript generates various products with different repertories of protein interaction modules. Three LIM-containing ENH predominates in neonatal cardiac tissue, whereas LIM-less ENHs are abundant in adult hearts, as well as skeletal muscles. Here we examine the timing of splicing transitions of ENH gene products during postnatal heart development and C2C12 myoblast differentiation. Real-time PCR analysis shows that LIM-containing ENH1 mRNA is gradually decreased during postnatal heart development, whereas transcripts with the short exon 5 appear in the late postnatal period and continues to increase until at least one month after birth. The splicing transition from LIM-containing ENH1 to LIM-less ENHs is also observed during the early period of C2C12 differentiation. This transition correlates with the emergence of ENH transcripts with the short exon 5, as well as the expression of myogenin mRNA. In contrast, the shift from the short exon 5 to the exon 7 occurs in the late differentiation period. The timing of this late event corresponds to the appearance of mRNA for the skeletal myosin heavy chain MYH4. Thus, coordinated and stepwise splicing transitions result in the production of specific ENH transcripts in mature striated muscles.
Volume 421(2)
Pages 232-8
Published 2012-5-4
DOI 10.1016/j.bbrc.2012.03.142
PII S0006-291X(12)00623-7
PMID 22497889
MeSH Adaptor Proteins, Signal Transducing / genetics* Animals Cell Differentiation / genetics Cell Line Gene Expression Regulation, Developmental* Heart / growth & development* Mice Microfilament Proteins / genetics* Muscle Development / genetics* Muscle, Striated / cytology Muscle, Striated / growth & development* Myoblasts, Cardiac / cytology RNA Splicing* Rats Rats, Sprague-Dawley
IF 2.985
Times Cited 6
Human and Animal Cells C2C12(RCB0987)