RRC ID 43785
Author Kimura K, Minamikawa Y, Ogasawara Y, Yoshida J, Saitoh K, Shinden H, Ye YQ, Takahashi S, Miyakawa T, Koshino H.
Title Kujigamberol, a new dinorlabdane diterpenoid isolated from 85million years old Kuji amber using a biotechnological assay.
Journal Fitoterapia
Abstract A new compound, 15,20-dinor-5,7,9-labdatriene-18-ol (1), named kujigamberol, was isolated from amber, fossilized tree resin from the Kuji area in Japan, has been dated as being 85 million years old (late Cretaceous). Kujigamberol was identified using the hypersensitive mutant yeast (zds1∆ erg3∆ pdr1∆ pdr3∆) with respect to Ca(2+)-signal transduction. The structure was elucidated on the basis of spectroscopic analysis including 1D NMR, 2D NMR and HR-EI-MS. It was different from known diterpenoids with a similar activity isolated from Baltic amber (agathic acid 15-monomethyl ester (2), dehydroabietic acid (3) and pimaric acid (4)). Kujigamberol showed glycogen synthase kinase-3β (GSK-3β) inhibition activity involving the growth restored activity against the mutant yeast and was cytotoxic to HL60 cells (IC(50)=19.6 μM).
Volume 83(5)
Pages 907-12
Published 2012-7-1
DOI 10.1016/j.fitote.2012.03.024
PII S0367-326X(12)00090-1
PMID 22507387
MeSH Amber / chemistry* Antineoplastic Agents, Phytogenic / isolation & purification* Antineoplastic Agents, Phytogenic / pharmacology Antineoplastic Agents, Phytogenic / therapeutic use Calcium / metabolism Diterpenes / isolation & purification* Diterpenes / pharmacology Diterpenes / therapeutic use Enzyme Inhibitors / isolation & purification* Enzyme Inhibitors / pharmacology Enzyme Inhibitors / therapeutic use Fossils* Glycogen Synthase Kinase 3 / antagonists & inhibitors Glycogen Synthase Kinase 3 beta HL-60 Cells Humans Japan Molecular Structure Phytotherapy* Plant Extracts / chemistry* Plant Extracts / pharmacology Plant Extracts / therapeutic use Signal Transduction Yeasts
IF 2.527
Times Cited 24
WOS Category PHARMACOLOGY & PHARMACY CHEMISTRY, MEDICINAL
Resource
Human and Animal Cells