| RRC ID |
43794
|
| 著者 |
Hokari Y, Seki T, Nakano H, Matsuo Y, Fukamizu A, Munekata E, Kiso Y, Mukai H.
|
| タイトル |
Isolation and identification of novel neutrophil-activating cryptides hidden in mitochondrial cytochrome C.
|
| ジャーナル |
Protein Pept Lett
|
| Abstract |
Although it is known that neutrophils infiltrate damaged sites immediately after tissue injury, the endogenous factors that induce their acute transmigration and activation have not been thoroughly investigated. For the candidates of those factors, we recently discovered two novel neutrophil-activating cryptides, mitocryptide-1 (MCT-1) and mitocryptide-2 (MCT-2), hidden in mitochondrial proteins. In addition, many unknown neutrophil-activating peptides other than MCT-1 and MCT-2 were also observed during their purification. Here, we isolated and purified a novel neutrophil-activating peptide from porcine hearts, which we showed by structural analyses to have an identical primary structure to porcine mitochondrial cytochrome c (68-85). We named this novel functional octadecapeptide as mitocryptide-CYC (MCT-CYC). Structure-activity relationships of cytochrome c on β-hexosaminidase (β-HA) release from neutrophilic-differentiated HL- 60 cells demonstrated that peptides derived from the C-terminal part of cytochrome c induced β-HA release and that cytochrome c (70-85) was the most potent cryptide among them. Since cytochrome c is known to be involved in the apoptotic process, our results suggest that cryptides, including MCT-CYC, derived from mitochondrial cytochrome c are possible factors that induce scavenging of toxic debris produced from apoptotic cells by neutrophils.
|
| 巻・号 |
19(6)
|
| ページ |
680-7
|
| 公開日 |
2012-6-1
|
| DOI |
10.2174/092986612800494048
|
| PII |
PPL-EPUB-20120418-014
|
| PMID |
22519541
|
| MeSH |
Amino Acid Sequence
Animals
Apoptosis
Chemokines / metabolism
Cytochromes c / chemistry*
Mitochondria, Heart / chemistry
Mitochondria, Heart / metabolism
Mitochondrial Proteins / chemistry*
Molecular Sequence Data
Myocardium / chemistry
Neutrophils / chemistry*
Neutrophils / metabolism
Peptides / chemistry*
Peptides / isolation & purification
Peptides / metabolism
Swine
beta-N-Acetylhexosaminidases / metabolism
|
| IF |
1.156
|
| 引用数 |
11
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HL60(RCB0041) |
