RRC ID 43795
Author Sakamoto N, Uemae Y, Ishikawa E, Takano S, Nakai K, Yamamoto T, Zaboronok A, Matsumura A.
Title Glioma immunotherapy with combined autologous tumor cell and endothelial cell vaccine in vivo.
Journal Neurol. Med. Chir. (Tokyo)
Abstract Combined vaccines containing GL261 murine glioma cells and F-2 murine endothelial cells fixed with glutaraldehyde-phosphate buffered saline were injected into the intradermal tissue of the tail base of C57BL/6 mice. After the vaccination, GL261 cells were injected subcutaneously into the left flank of the mice. Vaccination with fixed F-2 cells induced the development of relatively high amounts of interferon-gamma-releasing cells after in vitro re-stimulation with vascular endothelial growth factor-receptor 2 peptide. Tumor growth was inhibited after preventive use of the combined vaccine, prepared from GL261 and F-2 cells. Tumor specimens obtained from the combined vaccine group in a therapeutic experiment showed significantly decreased vessel count. Glioma immunotherapy with a combined vaccine prepared from tumor cells and endothelial cells might represent a new clinical strategy, as such combinations may theoretically affect both high-grade glioma cells and their environment.
Volume 52(4)
Pages 194-201
Published 2012
DOI 10.2176/nmc.52.194
PII JST.JSTAGE/nmc/52.194
PMID 22522329
MeSH Animals Brain Neoplasms / blood supply Brain Neoplasms / immunology Brain Neoplasms / therapy* Cancer Vaccines / immunology Cancer Vaccines / pharmacology* Cell Line, Tumor Cell Transplantation / methods* Glioma / blood supply Glioma / immunology Glioma / therapy* Immunotherapy / methods Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Neoplasm Transplantation / methods Neoplasms, Experimental / immunology Neoplasms, Experimental / physiopathology Neoplasms, Experimental / therapy* Primary Cell Culture Vaccines, Combined / immunology Vaccines, Combined / pharmacology*
IF 1.403
Times Cited 0
Human and Animal Cells